Proteome Interrogation Using Nanoprobes To Identify Targets of a Cancer-Killing Molecule

被引：18

作者：

Li, Liwen ^{[1
]}

Zhang, Qiu ^{[1
]}

Liu, Aifeng ^{[1
]}

Li, Xiue ^{[1
]}

Zhou, Hongyu ^{[2
]}

Liu, Yin ^{[1
]}

Yan, Bing ^{[1
,2
]}

机构：

[1] Shandong Univ, Sch Chem & Chem Engn, Minist Educ, Key Lab Colloid & Interface Chem, Jinan 250100, Peoples R China

[2] St Jude Childrens Hosp, Dept Chem Biol & Therapeut, Memphis, TN 38105 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 18期

基金：

中国国家自然科学基金;

关键词：

MICROTUBULE-INTERFERING AGENTS; ANTICANCER DRUGS; LUNG-CANCER; CELL-DEATH; HSP90; APOPTOSIS; TUBULIN; KINASE; IDENTIFICATION; COLCHICINE;

D O I：

10.1021/ja111137n

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We report a generic approach for identification of target proteins of therapeutic molecules using nanoprobes. Nanoprobes verify the integrity of nanoparticle-bound ligands in live cells and pull down target proteins from the cellular proteome, providing very important information on drug targets and mechanisms of action. As an example, target proteins as a-tubulin and HSP 90 have been identified and validated.

引用

页码：6886 / 6889

页数：4

共 24 条

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Roscovitine targets, protein kinases and pyridoxal kinase [J].