PEX19 is a predominantly cytosolic chaperone and import receptor for class 1 peroxisomal membrane proteins

被引:184
作者
Jones, JM [1 ]
Morrell, JC [1 ]
Gould, SJ [1 ]
机构
[1] Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA
关键词
peroxisome; protein import; postuanslational; Zellweger syndrome; PEX3;
D O I
10.1083/jcb.200304111
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Integral peroxisomal membrane proteins (PMPs) are synthesized in the cytoplasm and imported posttranslationally. Here, we demonstrate that PEX19 binds and stabilizes newly synthesized PMPs in the cytosol, binds to multiple PMP targeting signals (mPTSs), interacts with the hydrophobic domains of PMP targeting signals, and is essential for PMP targeting and import. These results show that PEX19 functions as both a chaperone and an import receptor for newly synthesized PMPs. We also demonstrate the existence of two PMP import mechanisms and two classes of mPTSs: class 1 mPTSs, which are bound by PEX19 and imported in a PEX19-dependent manner, and class 2 mPTSs, which are not bound by PEX19 and mediate protein import independently of PEX19.
引用
收藏
页码:57 / 67
页数:11
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