Monitoring of reactive oxygen species production after traumatic brain injury in rats with microdialysis and the 4-hydroxybenzoic acid trapping method

被引:66
作者
Marklund, N [1 ]
Clausen, F
Lewander, T
Hillered, L
机构
[1] Univ Uppsala Hosp, Dept Neurosci & Neurosurg, SE-75185 Uppsala, Sweden
[2] Univ Uppsala Hosp, Dept Psychiat, SE-75185 Uppsala, Sweden
[3] Univ Uppsala Hosp, Dept Med Sci, SE-75185 Uppsala, Sweden
关键词
free radicals; 2-hydroxybenzoic acid (salicylate : 2-HBA); 4-hydroxybenzoic acid (4-HBA); microdialysis; reactive oxygen species; traumatic brain injury (TBI);
D O I
10.1089/089771501317095250
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
The detection of reactive oxygen species (ROS) after traumatic brain injury (TBI) is based on indirect methods due to the high reactivity and short half-life of ROS in biological tissue. The commonly used salicylate trapping method has several disadvantages making it unsuitable for human use. We have evaluated 4-hydroxybenzoic acid (4-HBA) together with microdialysis (MD) in the rat as an alternative method. 4-HBA forms one stable adduct, 3,4-dihydroxybenzoic acid (3,4-DHBA), when reacting with ROS and has not previously been used together with MD after TBI. Twenty-seven rats were used for the assessment of 3,4-DHBA production as an indicator of ROS formation in a controlled contusion injury model using intracerebral MD with 3 mM 4-HBA in the perfusate. For comparison, salicylate trapping was used in eight rats. TBI caused a 250 % increase of 3,4-DHBA that peaked at 30 min after injury in severely injured rats and remained significantly elevated as compared to baseline for 90 min after trauma. The mild injury level caused a 100% increase in 3,4-DHBA formation at 30 min after the injury. When the MD probe was placed in the perimeter of the injury site, no significant increase in ROS formation occurred. Salicylate trapping showed a similar increase in adduct formation after severe injury. In addition, high cortical concentrations of 4-HBA and salicylate were found. It is concluded that microdialysis with 4-HBA as a trapping agent appears to be a useful method for ROS detection in the rat with a potential clinical utility.
引用
收藏
页码:1217 / 1227
页数:11
相关论文
共 53 条
  • [1] THE USE OF SALICYLATE HYDROXYLATION TO DETECT HYDROXYL RADICAL GENERATION IN ISCHEMIC AND TRAUMATIC BRAIN INJURY - REVERSAL BY TIRILAZAD MESYLATE (U-74006F)
    ALTHAUS, JS
    ANDRUS, PK
    WILLIAMS, CM
    VONVOIGTLANDER, PF
    CAZERS, AR
    HALL, ED
    [J]. MOLECULAR AND CHEMICAL NEUROPATHOLOGY, 1993, 20 (02) : 147 - 162
  • [2] THE MODE OF ACTION OF ASPIRIN-LIKE DRUGS - EFFECT ON INDUCIBLE NITRIC-OXIDE SYNTHASE
    AMIN, AR
    VYAS, P
    ATTUR, M
    LESZCZYNSKAPIZIAK, J
    PATEL, IR
    WEISSMANN, G
    ABRAMSON, SB
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) : 7926 - 7930
  • [3] Oxidative stress is attenuated in mice overexpressing BCL-2
    Bogdanov, MB
    Ferrante, RJ
    Mueller, G
    Ramos, LE
    Martinou, JC
    Beal, MF
    [J]. NEUROSCIENCE LETTERS, 1999, 262 (01) : 33 - 36
  • [4] ACTION OF SOME SALICYLATE DERIVATIVES ON INVITRO PLATELET-AGGREGATION - INHIBITORY AND INHIBITION ANTAGONISTIC EFFECTS
    CASADEBAIG, F
    DUPIN, JP
    GRAVIER, D
    HOU, G
    DARET, D
    BERNARD, H
    LARRUE, J
    BOISSEAU, M
    [J]. THROMBOSIS RESEARCH, 1991, 64 (06) : 631 - 636
  • [5] DEJANA E, 1983, THROMB RES, P153
  • [6] Increased free radical production due to subdural hematoma in the rat: Effect of increased inspired oxygen fraction
    Doppenberg, EMR
    Rice, MR
    Di, XO
    Young, HF
    Woodward, JJ
    Bullock, R
    [J]. JOURNAL OF NEUROTRAUMA, 1998, 15 (05) : 337 - 347
  • [7] The 21-aminosteroid U-74389G reduces cerebral superoxide anion concentration following fluid percussion injury of the brain
    Fabian, RH
    DeWitt, DS
    Kent, TA
    [J]. JOURNAL OF NEUROTRAUMA, 1998, 15 (06) : 433 - 440
  • [8] RESPONSES TO CORTICAL INJURY .1. METHODOLOGY AND LOCAL-EFFECTS OF CONTUSIONS IN THE RAT
    FEENEY, DM
    BOYESON, MG
    LINN, RT
    MURRAY, HM
    DAIL, WG
    [J]. BRAIN RESEARCH, 1981, 211 (01) : 67 - 77
  • [9] Anti-inflammatory activity in rats and mice of phenolic acids isolated from Scrophularia frutescens
    Fernández, MA
    Sáenz, MT
    García, MD
    [J]. JOURNAL OF PHARMACY AND PHARMACOLOGY, 1998, 50 (10) : 1183 - 1186
  • [10] FLOYD R A, 1986, Journal of Free Radicals in Biology and Medicine, V2, P13, DOI 10.1016/0748-5514(86)90118-2