The role of fluctuations in tRNA selection by the ribosome

被引：83

作者：

Lee, Tae-Hee

Blanchard, Scott C.

Kim, Harold D.

Puglisi, Joseph D. ^{[1
]}

Chu, Steven

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Phys, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[4] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA

[5] Univ Calif Berkeley, Dept Mol & Cell Biol, Lawrence Berkeley Lab, Berkeley, CA 94720 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 34期

关键词：

ribosome dynamics; ribosome selection; single-molecule FRET;

D O I：

10.1073/pnas.0705988104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The detailed mechanism of how the ribosome decodes protein sequence information with an abnormally high accuracy, after 40 years of study, remains elusive. A critical element in selecting correct transfer RNA (tRNA) transferring correct amino acid is '' induced fit '' between the ribosome and tRNA. By using single-molecule methods, the induced fit mechanism is shown to position favorably the correct tRNA after initial codon recognition. We provide evidence that this difference in positioning and thermal fluctuations constitutes the primary mechanism for the initial selection of tRNA. This work demonstrates thermal fluctuations playing a critical role in the substrate selection by an enzyme.

引用

页码：13661 / 13665

页数：5

共 29 条

[1] tRNA selection and kinetic proofreading in translation [J].