SRC transcriptional activation in a subset of human colon cancer cell lines

被引：43

作者：

Dehm, S

Senger, MA

Bonham, K ^{[1
]}

机构：

[1] Saskatchewan Canc Agcy, Hlth Res Div, Canc Res Unit, Saskatoon, SK S7N 4H4, Canada

[2] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7N 5E5, Canada

[3] Univ Saskatchewan, Div Oncol, Saskatoon, SK S7N 4H4, Canada

来源：

FEBS LETTERS | 2001年 / 487卷 / 03期

基金：

英国医学研究理事会;

关键词：

c-Src; oncogene activation; transcription; colon cancer;

D O I：

10.1016/S0014-5793(00)02354-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：367 / 371

页数：5

共 41 条

[1] DNA DISCONTINUITIES IN THE DOMAIN OF AMPLIFIED HUMAN MYC ONCOGENES [J].