Autolysis of Lactococcus lactis is influenced by proteolysis

被引:51
作者
Buist, G [1 ]
Venema, G [1 ]
Kok, J [1 ]
机构
[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Genet, NL-9751 NN Haren, Netherlands
关键词
D O I
10.1128/JB.180.22.5947-5953.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The autolysin AcmA of Lactococcus lactis was shown to be degraded by the extracellular Lactococcal proteinase PrtP. Autolysis, as evidenced by reduction in optical density of a stationary-phase culture and concomitant release of intracellular proteins, was greatly reduced when L. lactis MG1363 cells expressed the cell wall-anchored lactococcal proteinase PrtP of the PI-type caseinolytic specificity (PI). On the other hand, lactococcal strains that did not produce the proteinase showed a high level of autolysis, which was also observed when the cells produced the secreted form of PI or a cell wall-anchored proteinase with PIII-type specificity. Autolysis was also increased when MG1363 expressed the cell wall-anchored hybrid PI/PIII-type proteinase PIac. Zymographic analysis of AcmA activity during stationary phase showed that AcmA was quickly degraded by PI and much more slowly by PrtP proteinases with Pm-type and intermediate specificities. Autolysis oft. lactis by AcmA was influenced by the specificity, amount, and location of the lactococcal proteinase. No autolysis was observed when the various proteinases were expressed in an L. lactis acmA deletion mutant, indicating that PrtP itself did not cause lysis of cells. The chain length of a strain was significantly shortened when the strain expressed a cell wall-anchored active proteinase.
引用
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页码:5947 / 5953
页数:7
相关论文
共 34 条
  • [1] PLEIOTROPIC PHENOMENA IN AUTOLYTIC ENZYME(S) CONTENT, FLAGELLATION, AND SIMULTANEOUS HYPERPRODUCTION OF EXTRACELLULAR ALPHA-AMYLASE AND PROTEASE IN A BACILLUS-SUBTILIS MUTANT
    AYUSAWA, D
    YONEDA, Y
    YAMANE, K
    MARUO, B
    [J]. JOURNAL OF BACTERIOLOGY, 1975, 124 (01) : 459 - 469
  • [2] CLONING, SEQUENCING, AND EXPRESSION IN ESCHERICHIA-COLI OF A STREPTOCOCCUS-FAECALIS AUTOLYSIN
    BELIVEAU, C
    POTVIN, C
    TRUDEL, J
    ASSELIN, A
    BELLEMARE, G
    [J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (18) : 5619 - 5623
  • [3] Autolysis of Lactococcus lactis caused by induced overproduction of its major autolysin, AcmA
    Buist, G
    Karsens, H
    Nauta, A
    vanSinderen, D
    Venema, G
    Kok, J
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (07) : 2722 - 2728
  • [4] MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE MAJOR PEPTIDOGLYCAN HYDROLASE OF LACTOCOCCUS-LACTIS, A MURAMIDASE NEEDED FOR CELL-SEPARATION
    BUIST, G
    KOK, J
    LEENHOUTS, KJ
    DABROWSKA, M
    VENEMA, G
    HAANDRIKMAN, AJ
    [J]. JOURNAL OF BACTERIOLOGY, 1995, 177 (06) : 1554 - 1563
  • [5] BUIST G, 1997, THESIS U GRONINGEN G
  • [6] CHOPIN A, 1993, FEMS MICROBIOL REV, V12, P21, DOI [10.1016/0168-6445(93)90056-F, 10.1111/j.1574-6976.1993.tb00011.x]
  • [7] PROTEIN EXPORT DURING GROWTH OF BACILLUS-SUBTILIS - THE EFFECT OF EXTRACELLULAR PROTEASE DEFICIENCY
    COXON, RD
    HARWOOD, CR
    ARCHIBALD, AR
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1991, 12 (03) : 91 - 94
  • [8] CLONING AND EXPRESSION OF THE LACTOCOCCUS-LACTIS SUBSP CREMORIS SK11 GENE ENCODING AN EXTRACELLULAR SERINE PROTEINASE
    DEVOS, WM
    VOS, P
    DEHAARD, H
    BOERRIGTER, I
    [J]. GENE, 1989, 85 (01) : 169 - 176
  • [9] DIVERSITY OF CELL-ENVELOPE PROTEINASE SPECIFICITY AMONG STRAINS OF LACTOCOCCUS-LACTIS AND ITS RELATIONSHIP TO CHARGE CHARACTERISTICS OF THE SUBSTRATE-BINDING REGION
    EXTERKATE, FA
    ALTING, AC
    BRUINENBERG, PG
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (11) : 3640 - 3647
  • [10] GASSON MJ, 1983, J BACTERIOL, V154, P1