Alterations in expression of E2F-1 and E2F-responsive genes by RB, p53 and p21Sid1/WAF1/Cip1 expression

被引：11

作者：

Ookawa, K

Tsuchida, S

Kohno, T

Yokota, J

机构：

[1] Hirosaki Univ, Sch Med, Dept Biochem 2, Hirosaki, Aomori 0368562, Japan

[2] Natl Canc Ctr, Res Inst, Div Biol, Chuo Ku, Tokyo 1040045, Japan

来源：

FEBS LETTERS | 2001年 / 500卷 / 1-2期

关键词：

E2F-1; expression; RB; p21; p53;

D O I：

10.1016/S0014-5793(01)02583-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RB, p53 and p21(Sdi1/WAF1/Cip1) interact in the induction of G1 arrest, We established osteosarcoma cell lines in which a tetracycline-regulatable promoter controls the induction of RB, p53 and p21, By using these cell lines, we investigated whether RB, p53 or p21 regulates, in the same manner or differently, expression and function of E2F-1 and its responsive genes, E2F-1 gene products and transcripts of the E2F-responsive genes decreased in response to RB. Similar changes occurred to p53 and p21 when RB is present. However, in the absence of RB, some of the E2F-responsive genes decreased in response to p53 but not to p21, Thus, RB is a critical component for regulating the E2F-responsive genes, while p53 alone affects only a subset of these genes. (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V, All rights reserved.

引用

页码：25 / 30

页数：6

共 35 条

[1] Phenotypic alterations of small cell lung carcinoma induced by different levels of wild-type p53 expression [J].