Induction of β-1,3-Glucanase in Seeds of Maize Defective-Kernel Mutant (827Kpro1)

被引：6

作者：

Branco, Alan Trindade ^{[1
]}

Ferreira, Beatriz dos Santos ^{[1
]}

Lourenco, Guinevere Fernandes ^{[1
]}

Lopes Marques, Valeria Cristina ^{[5
]}

Tavares Machado, Olga Lima ^{[2
]}

Pereira, Messias Gonzaga ^{[3
]}

Aquino Almeida, Joao Carlos ^{[4
]}

de Souza Filho, Goncalo Apolinario ^{[1
]}

机构：

[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biotecnol, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[2] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Quim & Funcao Prot & Peptideos, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[3] Univ Estadual Norte Fluminense, Lab Melhoramento Genet Vegetal, Ctr Ciencias & Tecnol Agr, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[4] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Fisiol & Bioquim Microrganismos, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[5] Univ Estadual Norte Fluminense, Nucleo Anal Genom, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

来源：

PROTEIN AND PEPTIDE LETTERS | 2011年 / 18卷 / 07期

关键词：

beta-1,3-glucanase; defective kernel; dek; maize; seed; seed development; CLASS-I BETA-1,3-GLUCANASE; GENE-EXPRESSION PROFILES; MOLECULAR-BIOLOGY; METHYL JASMONATE; TOMATO SEEDS; PROTEINS; CHITINASE; ENDOSPERM; MORPHOGENESIS; ENCODES;

D O I：

10.2174/092986611795446012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-1,3-glucanases are found in organisms as diverse as plants, animals, bacteria and fungi. In plants, such enzymes are not only associated with defense mechanisms against pathogens, but also play critical roles in physiological and developmental processes. Here we identified a new beta-1,3-glucanase in maize seeds, and named it ZmGlucA. Sequence analysis revealed that ZmGlucA belongs to the class A of beta-1,3-glucanase, a class related to defense and physiological processes in plants. mRNA and protein assays showed that zmGlucA is expressed exclusively in seeds, and it is differentially regulated during seed development. Additionally, zmGlucA expression is strongly induced in seeds of the mutant dek 827Kpro1, which is defective for embryo and endosperm development. Our data support the idea that ZmGlucA protein is relevant to seed development.

引用

页码：651 / 657

页数：7

共 39 条

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