Rapid discrimination of environmental Vibrio by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

被引：34

作者：

Eddabra, Rkia ^{[1
]}

Prevost, Gilles ^{[1
]}

Scheftel, Jean-Michel ^{[1
]}

机构：

[1] Univ Strasbourg, UPRES EA 4438, Bacteriol Lab, Fac Med,Hop Univ Strasbourg, F-67000 Strasbourg, France

来源：

MICROBIOLOGICAL RESEARCH | 2012年 / 167卷 / 04期

关键词：

Antibiotic resistance; MALDI TOF; PFGE; Vibrio; Wastewater; FIELD GEL-ELECTROPHORESIS; GENOME SEQUENCE; CHROMOSOMAL DNA; IDENTIFICATION; RESISTANCE; CLASSIFICATION; STRAINS;

D O I：

10.1016/j.micres.2011.09.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to discriminate 30 Vibrio strains isolated from two wastewater treatment plants from Agadir, Morocco by two molecular typing methods, pulsed-field gel electrophoresis (PFGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Out of the 30 strains of Vibrio examined in this study, 5 isolates could not be typed by PFGE and consistently appeared as a smear on the gel. In general, high genetic biodiversity among the Vibrio strains was found regardless to the isolation source. The results of MALDI TOF analysis show a high congruence of strain grouping demonstrating the accuracy and reliability of MALDI-TOF MS. (C) 2011 Elsevier GmbH. All rights reserved.

引用

页码：226 / 230

页数：5

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