Retinal isomerization dynamics in dry bacteriorhodopsin films

被引：10

作者：

Colonna, A

Groma, GI

Vos, MH ^{[1
]}

机构：

[1] Ecole Polytech, CNRS, INSERM, Lab Opt Biosci,U696,UMR 7645,ENSTA, F-91128 Palaiseau, France

[2] Hungarian Acad Sci, Inst Biophys, Biol Res Ctr, H-6726 Szeged, Hungary

来源：

CHEMICAL PHYSICS LETTERS | 2005年 / 415卷 / 1-3期

基金：

匈牙利科学研究基金会;

关键词：

D O I：

10.1016/j.cplett.2005.08.132

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The primary photoprocesses in neutral and acid forms of oriented dried bacteriorhodopsin films were investigated by femtosecond absorption spectroscopy. The excitation energy dependence of the signals was used to distinguish photochemistry from processes involving photophysics of photocycle intermediates. Both the kinetics and the quantum yield of all-trans excited state decay by retinal photoisomerization and subsequent J -> K transition were found to be very similar as in hydrated environments. Therefore, unlike slower photocycle phases, communication of the retinal with the environment does not play a role in retinal isomerization. Our results are important for understanding recent nonlinear optical applications of such films. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：69 / 73

页数：5

共 33 条

[1] A COMPARISON OF THE 2ND-HARMONIC GENERATION FROM LIGHT-ADAPTED, DARK-ADAPTED, BLUE, AND ACID PURPLE MEMBRANE [J].