Differences between coding and non-coding regions in the Trichomonas vaginalis genome:: an actin gene as a locus model

被引：17

作者：

Espinosa, N

Hernández, R

López-Griego, L

Arroyo, R

López-Villaseñor, I

机构：

[1] Natl Autonomous Univ Mexico, Inst Invest Biomed, Dept Biotecnol, Mexico City 04510, DF, Mexico

[2] Natl Autonomous Univ Mexico, Inst Invest Biomed, Dept Biol Mol, Mexico City 04510, DF, Mexico

[3] IPN, CINVESTAV, Dept Expt Pathol, Mexico City 07300, DF, Mexico

来源：

ACTA TROPICA | 2001年 / 78卷 / 02期

关键词：

genome organization; G plus C content; open reading frames; adenylate cyclase; actin;

D O I：

10.1016/S0001-706X(00)00180-7

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The sequence of a cloned genomic fragment of Trichomonas vaginalis containing a complete actin gene was determined. An uninterrupted open reading frame of 1128 nucleotides was found that codes for an actin gene. Two overlapped consensus promoter sequences for T. vaginalis were found 12 nucleotides upstream the actin initiation codon. In addition to actin, two incomplete open reading frames were found at the 5' and 3' ends of the clone. These two sequences are expressed and showed similarity to adenylate cyclase genes and a yeast hypothetical protein. The overall sequence showed a higher G + C content and a lower frequency of repeated sequences in the coding regions when compared with the non-coding regions. A similar unequal nucleotide distribution was found in various T. vaginalis genes retrieved from data bases. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：147 / 154

页数：8

共 16 条

[1]

Bricheux G, 1997, FEMS MICROBIOL LETT, V153, P205, DOI 10.1111/j.1574-6968.1997.tb10483.x

[2] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].