X-ray diffraction on spider silk during controlled extrusion under a synchrotron radiation X-ray beam

被引:84
作者
Riekel, C
Madsen, B
Knight, D
Vollrath, F
机构
[1] European Synchrotron Radiat Facil, F-38043 Grenoble, France
[2] Aarhus Univ, Dept Zool, DK-8000 Aarhus C, Denmark
[3] Univ Oxford, Dept Zool, Oxford OX1 3PS, England
关键词
D O I
10.1021/bm000047c
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of a single thread of Nephila edulis silk has been studied by in situ X-ray diffraction from a living spider. A systematic increase of orientational order with increasing silking speed up to 40 mm s(-1) was observed. Within a few mm from the spinnerets exit, crystalline domains with a beta -poly(L-alanine) structure were observed. The data also suggest an increase in crystalline fraction in the immediate vicinity of the spigot exit.
引用
收藏
页码:622 / 626
页数:5
相关论文
共 21 条
[1]   STRUCTURE OF BETA-POLY-L-ALANINE - REFINED ATOMIC CO-ORDINATES FOR AN ANTI-PARALLEL BETA-PLEATED SHEET [J].
ARNOTT, S ;
DOVER, SD ;
ELLIOTT, A .
JOURNAL OF MOLECULAR BIOLOGY, 1967, 30 (01) :201-&
[2]   THE STRUCTURE AND PROPERTIES OF SPIDER SILK [J].
GOSLINE, JM ;
DEMONT, ME ;
DENNY, MW .
ENDEAVOUR, 1986, 10 (01) :37-43
[3]   Fiber morphology of spider silk: The effects of tensile deformation [J].
Grubb, DT ;
Jelinski, LW .
MACROMOLECULES, 1997, 30 (10) :2860-2867
[4]   Liquid crystals and flow elongation in a spider's silk production line [J].
Knight, DP ;
Vollrath, F .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1999, 266 (1418) :519-523
[5]   Beta transition and stress-induced phase separation in the spinning of spider dragline silk [J].
Knight, DP ;
Knight, MM ;
Vollrath, F .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2000, 27 (03) :205-210
[6]   Relations between elastic constants and the strain birefringence of high-elastic substances [J].
Kuhn, W ;
Grun, F .
KOLLOID-ZEITSCHRIFT, 1942, 101 (03) :248-271
[7]   Mechanics and morphology of silk drawn from anesthetized spiders [J].
Madsen, B ;
Vollrath, F .
NATURWISSENSCHAFTEN, 2000, 87 (03) :148-153
[8]  
NORTHOLT MG, 1991, ADV POLYM SCI, V98, P115
[9]   New avenues in x-ray microbeam experiments [J].
Riekel, C .
REPORTS ON PROGRESS IN PHYSICS, 2000, 63 (03) :233-262
[10]   In situ X-ray diffraction during forced silking of spider silk [J].
Riekel, C ;
Müller, M ;
Vollrath, F .
MACROMOLECULES, 1999, 32 (13) :4464-4466