The nine genes of the Nocardia lactamdurans cephamycin cluster are transcribed into large mRNAs from three promoters, two of them located in a bidirectional promoter region

被引：17

作者：

Enguita, FJ

Coque, JJR

Liras, P

Martin, JF ^{[1
]}

机构：

[1] Univ Leon, Fac Biol, Area Microbiol, E-24071 Leon, Spain

[2] INBIOTEC, Inst Biotechnol, E-24006 Leon, Spain

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 20期

关键词：

D O I：

10.1128/JB.180.20.5489-5494.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nine biosynthesis genes of the Nocardia lactamdurans cephamycin cluster are expressed as three different mRNAs initiating at promoters latp, cefDp, and pcbABp, as shown by low-resolution S1 nuclease protection assays and Northern blotting analysis. Bidirectional expression occurred from divergent promoters (latp and cefDp) located in a 629-bp intergenic region that contains three heptameric direct repeats similar to those recognized by members of the SARP (Streptomyces antibiotic regulatory proteins) family. The lat gene is transcribed in a single monocistronic transcript initiating at latp. A second unusually long polycistronic mRNA (more than 16 kb) corresponding to six biosynthesis genes (pcbAB, pcbC, cmcI, cmcJ, cefF and cmcH) started at pcbABp. A third polycistronic mRNA corresponding to the cefD and cefE genes started at cefDp.

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页码：5489 / 5494

页数：6

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