A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

被引：219

作者：

Ma, Dik-Lung ^{[1
]}

Wong, Wing-Leung ^{[1
]}

Chung, Wai-Hong ^{[1
]}

Chan, Fung-Yi ^{[1
]}

So, Pui-Kin ^{[1
]}

Lai, Tat-Shing ^{[1
]}

Zhou, Zhong-Yuan ^{[1
]}

Leung, Yun-Chung ^{[1
]}

Wong, Kwok-Yin ^{[1
]}

机构：

[1] Hong Kong Polytech Univ, Inst Mol Technol Drug Discovery & Synth, Cent Lab, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 20期

关键词：

electrophoresis; histidine; iridium; luminescent probes; protein staining;

D O I：

10.1002/anie.200705319

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3735 / 3739

页数：5

共 61 条

[1] A rapid solid-phase fluorescence-based protein assay for quantitation of protein electrophoresis samples containing detergents, chaotropes, dyes, and reducing agents [J].