LEA (late embryonic abundant)-like protein Hsp 12 (heat-shock protein 12) is present in the cell wall and enhances the barotolerance of the yeast Saccharomyces cerevisiae

被引:41
作者
Motshwene, P [1 ]
Karreman, R [1 ]
Kgari, G [1 ]
Brandt, W [1 ]
Lindsey, G [1 ]
机构
[1] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa
关键词
alkaline extraction; heat-shock protein 12 (Hsp 12); immunocytochemistry; late-embryonic-abundant-like protein; (LEA-like protein); Saccharomyces cerevisiae; yeast;
D O I
10.1042/bj20031301
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yeast cells Saccharomyces cerevisiae, late embryogenic abundant-like stress response protein Hsp 12 (heat-shock protein 12) were found by immunocytochemistry to be located both in the cytoplasm and in the cell wall, from where they could be extracted with dilute NaOH solutions. Yeast cells with the Hsp 12 gene disrupted were unable to grow in the presence of either 12 mM caffeine or 0.43 mM Congo Red, molecules known to affect cell-wall integrity. The volume of yeast cells were less affected by rapid changes in the osmolality of the growth medium when compared with the wild-type yeast cells, suggesting a role for Hsp 12 in the flexibility of the cell wall. This was also suggested by subjecting the yeast cells to rapid changes in barometric pressure where it was found that wild-type yeast cells were more resistant to cellular breakage.
引用
收藏
页码:769 / 774
页数:6
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