Profile and quantification of glucosinolates in Pentadiplandra brazzeana Baillon

被引:21
作者
De Nicola, Gina R. [2 ]
Nyegue, Maximilienne [3 ]
Montaut, Sabine [1 ]
Iori, Renato [2 ]
Menut, Chantal [4 ]
Tatibouet, Arnaud [5 ]
Rollin, Patrick [5 ]
Ndoye, Chantal [3 ]
Zollo, Paul-Henri Amvam [3 ]
机构
[1] Laurentian Univ, Dept Chem & Biochem, Sudbury, ON P3E 2C6, Canada
[2] Ind Crop Res Ctr CRA CIN, Agr Res Council, I-40128 Bologna, Italy
[3] Univ Yaounde I, Dept Biochem, Yaounde, Cameroon
[4] IBMM, UMR 5247, UM2, UM1, F-34093 Montpellier, France
[5] Univ Orleans, CNRS, UMR 6005, Inst Chim Organ & Analyt, F-45067 Orleans 2, France
基金
加拿大自然科学与工程研究理事会;
关键词
Pentadiplandra brazzeana; Pentadiplandraceae; Isolation; Desulfoglucosinolates; Desulfoglucotropaeolin; Desulfoglucolimnanthin; Desulfoglucoaubrietin; Isothiocyanates; PLANTS; PRODUCTS; ISOTHIOCYANATES; ETHNOBOTANY; MYROSINASE; DIVERSITY;
D O I
10.1016/j.phytochem.2011.09.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Glucosinolates (GLs) present in root, seed, and leaf extracts of Pentadiplandra brazzeana Baillon were characterized and quantified according to the ISO 9167-1 method based on the HPLC analysis of desulfo-GLs. The analyses were complemented by GC-MS analyses of the isothiocyanates (ITCs) generated from GL degradation by myrosinase. Glucotropaeolin (1a), glucolimnanthin (2a), and glucoaubrietin (3a) were shown to be present in the root extract, whereas the seed mainly contained 3a. 3,4-Dimethoxybenzyl GL (4a), glucobrassicin (5a) and traces of 1a were detected in the leaf extract. The products were fully characterized as their desulfo-counterparts by spectroscopic techniques. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:51 / 56
页数:6
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