Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes

被引:111
作者
China, B
Pirson, V
Mainil, J
机构
关键词
D O I
10.1128/AEM.62.9.3462-3465.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by sit genes, Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA(+), 15 were eneA(+) slt-I+, 1 was eneA(+) slt-I+ slt-II+, and 1 was eaeA(+) slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method.
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页码:3462 / 3465
页数:4
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