Integrated bioproduction and extraction of 3-methylcatechol

被引:36
作者
Hüsken, LE
Dalm, MCF
Tramper, J
Wery, J
de Bont, JAM
Beeftink, R
机构
[1] Univ Wageningen & Res Ctr, Dept Agrotechnol & Food Sci, Food & Bioproc Engn Grp, NL-6700 EV Wageningen, Netherlands
[2] Univ Wageningen & Res Ctr, Dept Agrotechnol & Food Sci, Div Ind Microbiol, NL-6700 EV Wageningen, Netherlands
关键词
Pseudomonas putida MC2; 3-methylcatechol production; optimization; organic/aqueous two-phase system; phase ratio;
D O I
10.1016/S0168-1656(01)00252-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pseudomonas putida MC2 is a solvent-tolerant strain that accumulates 3-methylcatechol. In aqueous media, 10 mM of 3-methylcatechol was produced and production was limited by 3-methylcatechol toxicity to the biocatalyst. Production levels increased by introduction of a second, organic phase that provides the substrate toluene and extracts the product from the culture medium. Octanol was shown to be an appropriate second phase with respect to tolerance of the strain for this solvent and with respect to partitioning of both substrate and product. Per unit of overall reactor volume (octanol and water), best results were obtained with 50% (v/v) of octanol: an overall 3-methylcatechol concentration of 25 mM was reached with 96% of the product present in the octanol phase. These product concentrations are much higher than in aqueous media without organic solvent, indicating that biocatalysis in an organic/aqueous two-phase system is an improved set-up for high production levels of 3-methylcatechol. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:11 / 19
页数:9
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