The Mycobacterium tuberculosis purine biosynthetic pathway: Isolation and characterization of the purC and purL genes

被引:26
作者
Jackson, M [1 ]
Berthet, FX [1 ]
Otal, I [1 ]
Rauzier, J [1 ]
Martin, C [1 ]
Gicquel, B [1 ]
Guilhot, C [1 ]
机构
[1] UNIV ZARAGOZA, FAC MED, DEPT MICROBIOL, E-50009 ZARAGOZA, SPAIN
来源
MICROBIOLOGY-SGM | 1996年 / 142卷
关键词
Mycobacterium tuberculosis; auxotrophy; complementation; purine biosynthetic pathway;
D O I
10.1099/00221287-142-9-2439
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Genes from the Mycobacterium tuberculosis purine biosynthetic pathway were identified using purine auxotrophic mutants of Mycobacterium smegmatis obtained by Tn611 transposon mutagenesis. Two approaches were followed in parallel. The first consisted of the complementation of the M. smegmatis purine auxotrophs using a M. tuberculosis H37Rv shuttle cosmid library. In the second approach, specific probes corresponding to the regions adjacent to the insertion sites of Tn611 in the M. smegmatis genome were used to screen a M. tuberculosis plasmid library by colony hybridization for inserts carrying homologous DNA fragments. Nucleotide sequence analysis of two M. tuberculosis genes isolated by these methods revealed high similarities with purC and purL genes from other bacterial and fungal sources, Transcriptional start sites were mapped for both genes, which revealed similar -10 boxes but with a higher GC content than the Escherichia coli sigma(70) consensus.
引用
收藏
页码:2439 / 2447
页数:9
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