Inhibition by heterologously-expressed P2Y(2) nucleotide receptors of N-type calcium currents in rat sympathetic neurones

被引：32

作者：

Filippov, AK

Webb, TE

Barnard, EA

Brown, DA

机构：

[1] UCL, DEPT PHARMACOL, LONDON WC1E 6BT, ENGLAND

[2] ROYAL FREE HOSP, SCH MED, MOL NEUROBIOL UNIT, LONDON NW3 2PF, ENGLAND

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1997年 / 121卷 / 05期

基金：

英国惠康基金;

关键词：

nucleotide receptors; P-2; receptors; uridine triphosphate; adenosine triphosphate; sympathetic neurones; calcium currents;

D O I：

10.1038/sj.bjp.0701270

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The P2Y(2) nucleotide receptor has previously been shown to stimulate phosphoinositide breakdown. We now show that, when P2Y(2) receptors are heterologously expressed by cRNA injection into dissociated rat sympathetic neurones, activation of these receptors by uridine 5'-triphosphate (UTP) or adenosine 5'-triphosphate (ATP) inhibits the N-type voltage-gated calcium current by similar to 65%, with an IC50 of 0.5 mu M. Thus, the same molecular species of nucleotide receptor can link to two different effector pathways.

引用

页码：849 / 851

页数：3

共 12 条

[1] Inhibition of N-type calcium channels. The only mechanism by which presynaptic alpha(2)-autoreceptors control sympathetic transmitter release [J].