Fluorescence microscopy today

被引:221
作者
Yuste, R [1 ]
机构
[1] Columbia Univ, Dept Biol Sci, Howard Hughes Med Inst, New York, NY 10027 USA
关键词
D O I
10.1038/nmeth1205-902
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescence microscopy has undergone a renaissance in the Last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in Living cells and of the structure and function of Living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear and hyperresolution microscopies are poised to profoundly transform many fields of biological research.
引用
收藏
页码:902 / 904
页数:3
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