Bacterial induction of pleural mesothelial monolayer barrier dysfunction

被引:50
作者
Mohammed, KA
Nasreen, N
Hardwick, J
Logie, CS
Patterson, CE
Antony, VB
机构
[1] Vet Affairs Med Ctr, Dept Med, Div Pulm & Crit Care Med, Indianapolis, IN 46202 USA
[2] Indiana Univ, Sch Med, Indianapolis, IN 46202 USA
关键词
vascular endothelial growth factor; mesothelial cells; protein permeability;
D O I
10.1152/ajplung.2001.281.1.L119
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Pneumonia remains one of the most common infectious causes of mortality. Patients with pneumonia develop parapneumonic effusions with a high neutrophil count as well as high protein concentrations. We hypothesized that pulmonary parenchymal bacterial infection causes a permeability change in the pleural mesothelium by inducing the production of vascular endothelial growth factor (VEGF). Complicated parapneumonic pleural effusions (empyema) have a 19-fold higher VEGF level than pleural fluids secondary to congestive heart failure and a 4-fold higher level than pleural fluids secondary to uncomplicated parapneumonic effusions. We also analyzed the influence of live Staphylococcus aureus on mesothelial barrier function using a model of confluent mesothelial monolayers. There was a significant drop in Electrical resistance across S. aureus-infected pleural mesothelial cell (PMC) monolayers. Recombinant VEGF also decreases PMC electrical resistance. Neutralizing antibodies to VEGF significantly inhibited the drop in PMC electrical resistance caused by S. aureus. S. aureus infection also caused a significant increase in protein leak across confluent mesothelial monolayers. Our results suggest that bacterial pathogens induce VEGF release in mesothelial cells and alter mesothelial permeability, leading to protein exudation in empyema.
引用
收藏
页码:L119 / L125
页数:7
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