Mutator phenotype resulting from DNA polymerase IV overproduction in Escherichia coli:: Preferential mutagenesis on the lagging strand

被引：40

作者：

Kuban, W

Banach-Orlowska, M

Bialoskorska, M

Lipowska, A

Schaaper, RM

Jonczyk, P

Fijalkowska, IJ

机构：

[1] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland

[2] NIEHS, Mol Genet Lab, Res Triangle Pk, NC 27709 USA

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 19期

关键词：

D O I：

10.1128/JB.187.19.6862-6866.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated the mutator effect resulting from overproduction of Escherichia coli DNA polymerase IV. Using lac mutational targets in the two possible orientations on the chromosome, we observed preferential mutagenesis during lagging strand synthesis. The mutator activity likely results from extension of mismatches produced by polymerase III holoenzyme.

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页码：6862 / 6866

页数：5

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