Developmental regulation of pectic epitopes during potato tuberisation

被引:76
作者
Bush, MS
Marry, M
Huxham, IM
Jarvis, MC
McCann, MC
机构
[1] John Innes Ctr Plant Sci Res, Dept Cell Biol, Norwich NR4 7UH, Norfolk, England
[2] Univ Glasgow, Integrated Microscopy Facil, Div Infect & Immun, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
cell wall; monoclonal antibody; pectin; Solanum; (development; pectin); tuberisation;
D O I
10.1007/s004250100570
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We show, by immunogold labelling, that potato (Solanum tuberosum L. cv Karnico) pectic epitopes are developmentally regulated within regions of the stolon, in addition to showing tissue-specific differences in abundance and localisation. The (1 -->4)-beta -D-galactan and (1 -->5)-alpha -arabinan epitopes demarcate two distinct zones within stolons; galactans are enriched in primary walls of elongating cells proximal to the stolon hook, whilst arabinans predominate in younger cells distal to the hook. Low-methoxyl homogalacturonan epitopes are concentrated in the middle lamella and show a proximo-distal gradient in stolons similar to that of galactans, whilst high-methoxyl homogalacturonan is uniformly abundant. Calcium pectate is restricted to the middle lamella at cell corners and pit fields. Calcium-binding sites are uniformly present in stolon cell walls, but their total density is reduced and they become localised to a few cell corners in mature tubers, as determined by image-electron energy loss spectroscopy. During the transition from elongation growth to ISO-diametric expansion during tuberisation of the stolon hook, there were no detectable changes in pectic epitope abundance or localisation. As tubers matured, all epitopes increased in abundance in parenchymal cell walls, except for calcium pectate. We conclude that potentially significant changes in pectic composition occur as young cells distal to the stolon hook move into the zone of cell elongation proximal to the hook.
引用
收藏
页码:869 / 880
页数:12
相关论文
共 51 条
[1]  
[Anonymous], C P PHYT 99
[2]   PECTINS AS MEDIATORS OF WALL POROSITY IN SOYBEAN CELLS [J].
BARONEPEL, O ;
GHARYAL, PK ;
SCHINDLER, M .
PLANTA, 1988, 175 (03) :389-395
[3]   Pectic epitopes are differentially distributed in the cell walls of potato (Solanum tuberosum) tubers [J].
Bush, MS ;
McCann, MC .
PHYSIOLOGIA PLANTARUM, 1999, 107 (02) :201-213
[4]   STRUCTURAL MODELS OF PRIMARY-CELL WALLS IN FLOWERING PLANTS - CONSISTENCY OF MOLECULAR-STRUCTURE WITH THE PHYSICAL-PROPERTIES OF THE WALLS DURING GROWTH [J].
CARPITA, NC ;
GIBEAUT, DM .
PLANT JOURNAL, 1993, 3 (01) :1-30
[5]   Assembly and enlargement of the primary cell wall in plants [J].
Cosgrove, DJ .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1997, 13 :171-201
[6]   Quantitative trait loci for polyamine content in an RFLP-mapped potato population and their relationship to tuberization [J].
Davies, PJ ;
Simko, I ;
Mueller, SM ;
Yencho, GC ;
Lewis, C ;
McMurry, S ;
Taylor, MA ;
Ewing, EE .
PHYSIOLOGIA PLANTARUM, 1999, 106 (02) :210-218
[7]   DEVELOPMENT OF ARABINANS AND GALACTANS DURING THE MATURATION OF HYPOCOTYL CELLS OF MUNG BEAN (VIGNA-RADIATA WILCZEK) [J].
DUPENHOAT, CH ;
MICHON, V ;
GOLDBERG, R .
CARBOHYDRATE RESEARCH, 1987, 165 (01) :31-42
[8]   Estimation of polymer rigidity in cell walls of growing and nongrowing celery collenchyma by solid-state nuclear magnetic resonance in vivo [J].
Fenwick, KM ;
Jarvis, MC ;
Apperley, DC .
PLANT PHYSIOLOGY, 1997, 115 (02) :587-592
[9]   The pore size of non-graminaceous plant cell walls is rapidly decreased by borate ester cross-linking of the pectic polysaccharide rhamnogalacturonan II [J].
Fleischer, A ;
O'Neill, MA ;
Ehwald, R .
PLANT PHYSIOLOGY, 1999, 121 (03) :829-838
[10]  
Foster TJ, 1996, BIOPOLYMERS, V39, P51, DOI 10.1002/(SICI)1097-0282(199607)39:1<51::AID-BIP6>3.3.CO