Production of human lactoferrin in transgenic tobacco plants

被引：62

作者：

Salmon, V

Legrand, D

Slomianny, MC

El Yazidi, I

Spik, G ^{[1
]}

Gruber, V

Bournat, P

Olagnier, B

Mison, D

Theisen, M

Mérot, B

机构：

[1] Univ Sci & Tech Lille Flandres Artois, Chim Biol Lab, F-59655 Villeneuve Dascq, France

[2] Univ Sci & Tech Lille Flandres Artois, CNRS, Unite Mixte Rech 111, F-59655 Villeneuve Dascq, France

[3] MERISTEM Therapeut, F-63100 Clermont Ferrand, France

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1998年 / 13卷 / 01期

关键词：

D O I：

10.1006/prep.1998.0886

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Production and characterization of human lactoferrin (hLf) in transgenic tobacco is reported. We have engineered two constructs containing either the native signal peptide from human lactoferrin or the signal peptide from sweet potato sporamin fused to human lactoferrin encoding cDNA. N-terminal sequences of rhLf purified from tobacco were identical to Lf from human milk for both constructs. The tobacco rhLf presents a molecular mass closely identical to native protein. Overall sugar composition shows the presence of plant specific xylose while sialic acid is absent. Binding parameters of the recombinant molecule to both Jurkat lymphoblastic T-cells or HT29-18-C1 enterocytes are similar to those of human lactoferrin isolated from milk. (C) 1998 Academic Press.

引用

页码：127 / 135

页数：9

共 43 条

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