Determination of the human salivary peptides histatins 1, 3, 5 and statherin by high-performance liquid chromatography and by diode-array detection

被引：33

作者：

Castagnola, M

Congiu, D

Denotti, G

Di Nunzio, A

Fadda, MB

Melis, S

Messana, I

Misiti, F

Murtas, R

Olianas, A

Piras, V

Pittau, A

Puddu, G

机构：

[1] Univ Cagliari, Dept Sci Appl Biosys, Sect Biochem & Mol Biol, I-09042 Cagliari, Italy

[2] Ctr Chem Receptor & Biol Act Mol, CNR, Rome, Italy

[3] Univ Cagliari, Dept Odontostomatol, Cagliari, Italy

[4] Catholic Univ Rome, Fac Med, Inst Chem & Clin Chem, Rome, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 751卷 / 01期

关键词：

peptides; histatins; statherin;

D O I：

10.1016/S0378-4347(00)00466-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatography (HPLC) method with diode-array detection for the quantification of several human salivary peptides is described. Sample pretreatment consisted of the acidification of whole saliva by phosphate buffer. This treatment produced precipitation of mucins, cu-amylases and other high-molecular-mass salivary proteins, simultaneous inhibition of intrinsic protease activities and reduction of sample viscosity. Direct HPLC analysis by diode-array detection of the resulting acidic sample allowed one to quantify histatin 1, histatin 3, histatin 5, statherin, as well as uric acid, in normal subjects. Moreover, the groups of peaks pertaining to proline-rich proteins and cystatins were tentatively identified. The method can be useful in assessing the concentration of salivary peptides from normal subjects and from patients suffering oral and/or periodontal diseases. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 160

页数：8

共 29 条

[11] CHARACTERIZATION AND AMINO-ACID-SEQUENCE OF A NEW ACIDIC CYSTEINE PROTEINASE-INHIBITOR (CYSTATIN-SA) STRUCTURALLY CLOSELY RELATED TO CYSTATIN-S, FROM HUMAN WHOLE SALIVA [J].