Wiring an [FeFe]-Hydrogenase with Photosystem I for Light-Induced Hydrogen Production

被引：94

作者：

Lubner, Carolyn E. ^{[1
]}

Knoerzer, Philipp ^{[2
]}

Silva, Paulo J. N. ^{[3
]}

Vincent, Kylie A. ^{[4
]}

Happe, Thomas ^{[2
]}

Bryant, Donald A. ^{[3
]}

Golbeck, John H. ^{[3
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Ruhr Univ Bochum, Lehrstuhl Biochem Pflanzen, AG Photobiotechnol, D-44780 Bochum, Germany

[3] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

[4] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England

来源：

BIOCHEMISTRY | 2010年 / 49卷 / 48期

基金：

美国国家科学基金会;

关键词：

PHOTOCATALYTIC PRODUCTION; MODIFIED LIGANDS; F-A; RECONSTITUTION; COMPLEX;

D O I：

10.1021/bi1016167

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A molecular wire is used to connect two proteins through their physiologically relevant redox cofactors to facilitate direct electron transfer. Photosystem I (PS I) and an [FeFe]-hydrogenase (H(2)ase) serve as the test bed for this new technology. By tethering a photosensitizer with a hydrogen-evolving catalyst, attached by Fe-S coordination bonds between the F-B iron-sulfur cluster of PS 1 and the distal iron-sulfur cluster of H(2)ase, we assayed electron transfer between the two components via light-induced hydrogen generation. These hydrogen-producing nanoconstructs self-assemble when the PS I variant, the H(2)ase variant, and the molecular wire are combined.

引用

页码：10264 / 10266

页数：3

共 21 条

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