Levels of endogenous cytokinins, indole-3-acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY-2 cells

被引:116
作者
Redig, P
Shaul, O
Inze, D
VanMontagu, M
VanOnckelen, H
机构
[1] UNIV INSTELLING ANTWERP, DEPT BIOL, B-2610 ANTWERP, BELGIUM
[2] STATE UNIV GHENT VIB, INST BIOTECHNOL, LAB GENET, B-9000 GHENT, BELGIUM
[3] STATE UNIV GHENT, LAB ASSOCIE INST NATL RECH AGRON FRANCE, B-9000 GHENT, BELGIUM
来源
FEBS LETTERS | 1996年 / 391卷 / 1-2期
关键词
cell cycle; cytokinin; mass spectrometry; plant hormone; synchronization; tobacco BY-2 cell;
D O I
10.1016/0014-5793(96)00728-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Correlation between cell cycle progression and endogenous levels of plant hormones was studied in synchronized tobacco BY-2 cell suspension cultures. Sixteen different cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were extracted using solid-phase anion exchange chromatography in combination with immunoaffinity purification, and quantified by mass spectrometry. No significant correlation could be identified for IAA and ABA, In contrast, there were sharp peaks in the levels of specific cytokinins (zeatin- and dihydrozeatin-type) at the end of the S phase and during mitosis. The levels of other cytokinins analyzed, including zeatins N- and O-glucosides, remained low, suggesting that the increased amounts of their corresponding non-glucosylated form resulted from de novo synthesis. These findings suggest that zeatin- and dihydrozeatin-type cytokinins might play a specific regulatory role in the progression of the plant cell cycle. One hypothesis to explain cytokinin action is based on a specific interaction with kinases that regulate cell cycle progression, as has been recently shown for the cytokinin analogue olomoucine.
引用
收藏
页码:175 / 180
页数:6
相关论文
共 36 条
[1]   EFFECT OF ETHYLENE ON CELL-DIVISION AND DEOXYRIBONUCLEIC ACID SYNTHESIS IN PISUM-SATIVUM [J].
APELBAUM, A ;
BURG, SP .
PLANT PHYSIOLOGY, 1972, 50 (01) :117-+
[2]  
BANERJEE SN, 1968, PLANT CELL PHYSIOL, V9, P577
[3]   THE EFFECT OF ABSCISIC-ACID ON CELL-GROWTH, CELL-DIVISION AND DNA-SYNTHESIS IN THE MAIZE ROOT-MERISTEM [J].
BARLOW, PW ;
PILET, PE .
PHYSIOLOGIA PLANTARUM, 1984, 62 (02) :125-132
[4]   PROBLEM OF HALTING ENZYME ACTION WHEN EXTRACTING PLANT TISSUES [J].
BIELESKI, RL .
ANALYTICAL BIOCHEMISTRY, 1964, 9 (04) :431-&
[5]   HORMONAL-CONTROL OF TOBACCO PROTOPLAST NUCLEIC-ACID METABOLISM DURING INVITRO CULTURE [J].
COOKE, R ;
MEYER, Y .
PLANTA, 1981, 152 (01) :1-7
[6]   INHIBITION OF GROWTH OF CULTURED TOBACCO CELLS AT LOW CONCENTRATIONS OF LOVASTATIN IS REVERSED BY CYTOKININ [J].
CROWELL, DN ;
SALAZ, MS .
PLANT PHYSIOLOGY, 1992, 100 (04) :2090-2095
[7]  
DAVIES PJ, 1995, PLANT HORMONES PHYSL, P7
[8]   POLYRIBOSOME FORMATION IN RELATION TO CYTOKININ-INDUCED CELL-DIVISION IN SUSPENSION CULTURES OF GLYCINE-MAX [L] MERR [J].
FOSKET, DE ;
VOLK, MJ ;
GOLDSMITH, MR .
PLANT PHYSIOLOGY, 1977, 60 (04) :554-562
[9]   OLOMOUCINE, AN INHIBITOR OF THE CDC2/CDK2 KINASES ACTIVITY, BLOCKS PLANT-CELLS AT THE G1 TO S AND G2 TO M CELL-CYCLE TRANSITIONS [J].
GLAB, N ;
LABIDI, B ;
QIN, LX ;
TREHIN, C ;
BERGOUNIOUX, C ;
MEIJER, L .
FEBS LETTERS, 1994, 353 (02) :207-211
[10]   INFLUENCE OF MYCORRHIZAE AND RHIZOBIUM ON CYTOKININ CONTENT IN DROUGHT-STRESSED ALFALFA [J].
GOICOECHEA, N ;
DOLEZAL, K ;
ANTOLIN, MC ;
STRNAD, M ;
SANCHEZDIAZ, M .
JOURNAL OF EXPERIMENTAL BOTANY, 1995, 46 (291) :1543-1549