An optogenetic toolbox designed for primates

被引：314

作者：

Diester, Ilka ^{[1
]}

Kaufman, Matthew T. ^{[2
]}

Mogri, Murtaza ^{[1
]}

Pashaie, Ramin ^{[1
]}

Goo, Werapong ^{[1
]}

Yizhar, Ofer ^{[1
]}

Ramakrishnan, Charu ^{[1
]}

Deisseroth, Karl ^{[1
,2
,3
,4
]}

Shenoy, Krishna V. ^{[1
,2
,5
]}

机构：

[1] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[2] Stanford Univ, Neurosci Program, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Psychiat & Behav Sci, Stanford, CA 94305 USA

[4] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

[5] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA

来源：

NATURE NEUROSCIENCE | 2011年 / 14卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

SENSORY-MOTOR CORTEX; IN-VIVO CONTROL; OPTICAL CONTROL; MILLISECOND-TIMESCALE; ADENOASSOCIATED VIRUS; CORTICAL-NEURONS; NEURAL CIRCUITRY; NONHUMAN PRIMATE; GENE-THERAPY; MONKEY;

D O I：

10.1038/nn.2749

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Optogenetics is a technique for controlling subpopulations of neurons in the intact brain using light. This technique has the potential to enhance basic systems neuroscience research and to inform the mechanisms and treatment of brain injury and disease. Before launching large-scale primate studies, the method needs to be further characterized and adapted for use in the primate brain. We assessed the safety and efficiency of two viral vector systems (lentivirus and adeno-associated virus), two human promoters (human synapsin (hSyn) and human thymocyte-1 (hThy-1)) and three excitatory and inhibitory mammalian codon-optimized opsins (channelrhodopsin-2, enhanced Natronomonas pharaonis halorhodopsin and the step-function opsin), which we characterized electrophysiologically, histologically and behaviorally in rhesus monkeys (Macaca mulatta). We also introduced a new device for measuring in vivo fluorescence over time, allowing minimally invasive assessment of construct expression in the intact brain. We present a set of optogenetic tools designed for optogenetic experiments in the non-human primate brain.

引用

页码：387 / 397

页数：11

共 49 条

[1] Neural substrates of awakening probed with optogenetic control of hypocretin neurons [J].