The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin

被引：30

作者：

Chen, B ^{[1
]}

Bestetti, G ^{[1
]}

Day, RM ^{[1
]}

Turner, APF ^{[1
]}

机构：

[1] Cranfield Univ, Cranfield Biotechnol Ctr, Cranfield MK43 0AL, Beds, England

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 7-8期

基金：

英国生物技术与生命科学研究理事会;

关键词：

affinity ligand; combinatorial peptide library; computer modelling simulation; diabetes; glycated proteins; glycosylated haemoglobin; one-bead one-compound;

D O I：

10.1016/S0956-5663(98)00042-6

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA(1c)), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA(1c). The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：779 / 785

页数：7

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