Polymorphism in morels: Isozyme electrophoretic analysis

被引：9

作者：

Wipf, D ^{[1
]}

Bedell, JP ^{[1
]}

Munch, JC ^{[1
]}

Botton, B ^{[1
]}

Buscot, F ^{[1
]}

机构：

[1] BUNDESFORSCH ANSTALT LANDWIRTSCHAFT,INST BODENBIOL,D-38116 BRAUNSCHWEIG,GERMANY

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1996年 / 42卷 / 08期

关键词：

isozyme analysis; electrophoresis; Morchella sp; polymorphism;

D O I：

10.1139/m96-103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to assess whether isozyme polymorphism in different members of the Morchellaceae could be used to improve the systematics in this fungal group and to characterize intraspecific crossings between monosporal strains in Morchella esculenta. For this purpose, isozyme electrophoretic analysis of the following enzymes was performed: glutamine synthetase, NAD-glutamate dehydrogenase, NADP-glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase, NAD-glyceraldehyde phosphate dehydrogenase, glucose phosphate isomerase, and superoxide dismutase. The analyses allowed discrimination at the inter- or intra-specific levels and could help to establish a method of identification for strains in the Morchellaceae. To a certain extent they appeared to be suitable to analyze interactions of monosporal strains of Morchella esculenta in pairing experiments. The polymorphism shown in this study was consistent with the phylogenetic relationships between the investigated strains only at the genus level.

引用

页码：819 / 827

页数：9

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