Animal and human pathogenic Escherichia coli strains share common genetic backgrounds

被引:170
作者
Clermont, Olivier [1 ,2 ]
Olier, Maiwenn [3 ]
Hoede, Claire [1 ,2 ]
Diancourt, Laure [4 ]
Brisse, Sylvain [4 ]
Keroudean, Monique [3 ]
Glodt, Jeremy [1 ,2 ]
Picard, Bertrand [1 ,5 ]
Oswald, Eric [3 ,6 ]
Denamur, Erick [1 ,2 ]
机构
[1] INSERM, UMR722, F-75018 Paris, France
[2] Univ Paris Diderot, F-75018 Paris, France
[3] INRA, UMR 1225, F-31076 Toulouse, France
[4] Inst Pasteur, F-75724 Paris, France
[5] Univ Paris 13, F-75018 Paris, France
[6] CHU Toulouse, Lab Bacteriol Hyg, Inst Fed Biol, F-31059 Toulouse, France
关键词
Escherichia coli; Phylogeny; Host specificity; Pathogenic; HEMOLYTIC-UREMIC SYNDROME; PHYLOGENETIC ANALYSIS; VIRULENCE FACTORS; ANTIMICROBIAL RESISTANCE; PARALLEL EVOLUTION; HOST-SPECIFICITY; IDENTIFICATION; DIVERSITY; COMMENSAL; HETEROGENEITY;
D O I
10.1016/j.meegid.2011.02.005
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Escherichia coli is a versatile species encompassing both commensals of the digestive tracts of many vertebrates, including humans, and pathogenic strains causing various intra- and extraintestinal infections. Despite extensive gene flow between strains, the E. coli species has a globally clonal population structure, consisting of distinct phylogenetic groups. Little is known about the relationships between phylogenetic groups and host specificity. We therefore used multilocus sequence typing (MLST) to investigate phylogenetic relationships and evaluated the virulence gene content of 35 E. coli strains representative of the diverse diseases encountered in domestic animals. We compared these strains with a panel of 101 human pathogenic and 98 non-human and human commensal strains representative of the phylogenetic and pathovar diversity of this species. A global factorial analysis of correspondence indicated that extraintestinal infections were caused mostly by phylogenetic group B2 strains, whereas intraintestinal infections were caused mostly by phylogenetic group A/B1/E strains, with strains responsible from extraintestinal or intraintestinal infections having specific virulence factors. It was not possible to distinguish between strains of human and animal origin. A detailed phylogenetic analysis of the MLST data showed that numerous pathogenic animal and human strains are very closely related, and had a number of virulence genes in common. However, a set of specific adhesins was identified in animal non-B2 group strains of all pathotypes. In conclusion, human and animal pathogenic strains share common genetic backgrounds, but non-B2 strains of different origins seem to have different sets of adhesins that could be involved in host specificity. (c) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:654 / 662
页数:9
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