Proteome Analysis of Differential Protein Expression in Brain of Rats with Type 1 Diabetes Mellitus

Aim of the study: Proteomic approach was applied to identify differential protein expressed in brain of rats with type 1 diabetes mellitus (T1DM) in order to search for potential biomarkers for pathological changes of brain with T1DM. Methods: Proteins were extracted from brain tissues of T1DM rats and healthy control rats, separated by 2-DE and identified by MALDI-TOF-MS. The results were validated by Western blot analysis and immunohistochemistry (IHC). Results: A total of 8 proteins from the 24 differentially expressed spots were identified by MALDI-TOF-MS. The proteins identified were vitamin D-dependent calcium-binding protein, creatine kinase B-type (B-CK), myosin light chain kinase (MLCK), HSP60 and HSP71, ATP synthase, cyclin-G, pantothenate kinase-1 (PANK1), respectively. 3 proteins were up-regulated and 5 proteins were down-regulated from the T1DM rats. Of the 8 proteins identified, MLCK was confirmed by Western blot and IHC. Conclusion: This work demonstrates that a comprehensive strategy of proteomic identification should be a useful tool for understanding of diabetic encephalopathy mechanism. And the differential proteins such as MLCK may be give clues about the pathogenesis of diabetic encephalopathy.