Inhibitive effect of triptolide on invasiveness of human fibrosarcoma cells by downregulating matrix metalloproteinase-9 expression

被引：18

作者：

Yang, Shengbo ^{[1
]}

Gu, Can ^{[2
]}

Zhang, Guiying ^{[3
]}

Kang, Jian ^{[1
]}

Wen, Haiquan ^{[3
]}

Lu, Qianjin ^{[3
]}

Huang, Jinhua ^{[1
]}

机构：

[1] Cent S Univ, Xiangya Hosp 3, Dept Dermatol, Changsha 410013, Hunan, Peoples R China

[2] Cent S Univ, Sch Nursing, Changsha 410013, Hunan, Peoples R China

[3] Cent S Univ, Xiangya Hosp 2, Dept Dermatol, Changsha 410011, Hunan, Peoples R China

来源：

ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2011年 / 4卷 / 06期

关键词：

Triptolide; Matrix metalloproteinase; Fibrosarcoma; Neoplasm metastasis; WILFORDII HOOK-F; GENE-EXPRESSION; MATRIX METALLOPROTEINASES; FIBROBLASTS; METASTASIS; SUPPRESSES; APOPTOSIS; DEATH;

D O I：

10.1016/S1995-7645(11)60131-4

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

100235 [预防医学];

摘要：

Objective: To explore the molecular mechanisms of antitumor properties of triptolide, a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook P. Methods: Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h. Then the expression and activity of matrix metalloproteinase (MMP)-2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9-treated HT-1080 cells. Results: 18 nmol/L triptolide inhibited the gene expression and activity of MMP-9, but not those of MMP-2, in HT-1080 cells. In addition, both 18 nmol/L triptolide and 3 mu g/mL anti-MMP-9 significantly reduced the invasive potential of HT-1080 cells, by about 50% and 35%, respectively, compared with die control. Whereas there was no significant difference between the effect of 18 nmol/L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells. Conclusions: These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.

引用

页码：482 / 485

页数：4

共 21 条

[1]

DIRECT EVIDENCE LINKING EXPRESSION OF MATRIX METALLOPROTEINASE-9 (92-KDA GELATINASE/COLLAGENASE) TO THE METASTATIC PHENOTYPE IN TRANSFORMED RAT EMBRYO CELLS [J].