Isolation, characterization, and molecular identification of bacteria from the red imported fire ant (Solenopsis invicta) midgut

被引：57

作者：

Li, HW ^{[1
]}

Medina, F ^{[1
]}

Vinson, SB ^{[1
]}

Coates, CJ ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2005年 / 89卷 / 03期

关键词：

red imported fire ant; Solenopsis invicta; 16s rRNA gene; biological control; bacteria;

D O I：

10.1016/j.jip.2005.05.008

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Bacteria were isolated and cultured from the red imported fire ant (Solenopsis invicta) midgut. The small-subunit ribosomal RNA gene, (16s rRNA gene, approximately 1500 bp) was amplified from bacterial genomic DNA using the polymerase chain reaction and consensus sequence primers. Restriction fragment length polymorphisin analysis revealed 10 unique profiles, indicating that at least 10 different bacteria are present in red imported fire ant midguts. The 16s rRNA gene sequence was determined for these isolates and queried against the NCBI genetic database. The results identified all isolates to at least the genus level. Antibiotic resistance profiles and biochemical activities were also determined for these species. This work provides the basis for a wider characterization of bacterial distributions in fire ant colonies and provides strains suitable for genetic manipulation to develop novel methods of fire ant control. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：203 / 209

页数：7

共 44 条

[1] Garden sharing and garden stealing in fungus-growing ants [J].