Adsorption of essential micronutrients by carbon nanotubes and the implications for nanotoxicity testing

被引：137

作者：

Guo, Lin ^{[2
]}

Von Dem Bussche, Annette ^{[1
]}

Buechner, Michelle ^{[1
]}

Yan, Aihui ^{[3
]}

Kane, Agnes B. ^{[1
]}

Hurt, Robert H. ^{[2
]}

机构：

[1] Brown Univ, Dept Pathol & Lab Med, Providence, RI 02912 USA

[2] Brown Univ, Div Engn, Providence, RI 02912 USA

[3] Brown Univ, Dept Chem, Providence, RI 02912 USA

来源：

SMALL | 2008年 / 4卷 / 06期

关键词：

amino acids; biological activity; carbon nanotubes; toxicology; vitamins;

D O I：

10.1002/smll.200700754

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A study used biochemical profiling techniques and ultraviolet (UV) visible spectroscopy, to demonstrate that single-walled nanotubes (SWNTs) caused dose-dependent adsorption and depletion of more than 14 amino acids and vitamins from RPMI cell culture medium. It was found that HepG2 cells cultured through these techniques, showed significantly reduced viability that can be restored by replenishment of folate. The study demonstrated a new mechanism through which hydrophobic nanotube formulations influenced cell behavior indirectly. The study involved the dispersion of SWNTs in RPMI cell culture medium at different doses and sonicating for 30 minutes, to conduct investigations.

引用

页码：721 / 727

页数：7

共 33 条

[1]

[Anonymous], 2006, ANGEW CHEM, DOI DOI 10.1002/smll.201202636

[2] DEVELOPMENT OF HYDROPHOBICITY PARAMETERS TO ANALYZE PROTEINS WHICH BEAR POSTTRANSLATIONAL OR COTRANSLATIONAL MODIFICATIONS [J].