Passive electrotonic properties of rat hippocampal CA3 interneurones

被引:62
作者
Chitwood, RA [1 ]
Hubbard, A [1 ]
Jaffe, DB [1 ]
机构
[1] Univ Texas, Div Life Sci, San Antonio, TX 78249 USA
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1999年 / 515卷 / 03期
关键词
D O I
10.1111/j.1469-7793.1999.743ab.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1.The linear membrane responses of CA3 interneurones were determined with the use of whole-cell patch recording methods. The mean input resistance (R-N) for all cells in this study was 526 +/- 16 M Omega and the slowest membrane time constant (tau(0)) was 73 +/- 3 ms. 2. The three-dimensional morphology of 63 biocytin-labelled neurones was used to construct compartmental models. Specific membrane resistivity (R-m) and specific membrane capacitance (C-m) were estimated by fitting the linear membrane response. Acceptable fits were obtained for 24 CA3 interneurones. The mean R-m was 61.9 +/- 34.2 Ohm cm(2) and the mean C-m was 0.9 +/- 0.3 mu F cm(-2). Intracellular resistance (R-i) could not be resolved in this study. 3. Examination of voltage attenuation revealed a significantly low synaptic efficiency from most dendritic synaptic input locations to the soma. 4. Simulations of excitatory postsynaptic potentials (EPSPs) were analysed at both the site of synaptic input and at the soma. There was little variability in the depolarization at the soma from synaptic inputs placed at different locations along the dendritic tree. The EPSP amplitude at the site of synaptic input was progressively larger with distance from the soma, consistent with a progressive increase in input impedance. 5. The 'iso-efficiency' of spatially different synaptic inputs arose from two opposing factors: an increase in EPSP amplitude at the synapse with distance from the soma was opposed by a nearly equivalent increase in voltage attenuation. These simulations suggest that, in these particular neurones, the amplitude of EPSPs measured at the soma will not be significantly affected by the location of synaptic inputs.
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收藏
页码:743 / 756
页数:14
相关论文
共 47 条
  • [1] CA1 pyramidal to basket and bistratified cell EPSPs: dual intracellular recordings in rat hippocampal slices
    Ali, AB
    Deuchars, J
    Pawelzik, H
    Thomson, AM
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1998, 507 (01): : 201 - 217
  • [2] EXCITATORY SYNAPTIC CONNECTIONS ONTO RAT HIPPOCAMPAL INHIBITORY CELLS MAY INVOLVE A SINGLE TRANSMITTER RELEASE SITE
    ARANCIO, O
    KORN, H
    GULYAS, A
    FREUND, T
    MILES, R
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1994, 481 (02): : 395 - 405
  • [3] AMPLIFICATION AND LINEARIZATION OF DISTAL SYNAPTIC INPUT TO CORTICAL PYRAMIDAL CELLS
    BERNANDER, O
    KOCH, C
    DOUGLAS, RJ
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1994, 72 (06) : 2743 - 2753
  • [4] ELECTRO-PHYSIOLOGICAL CHARACTERIZATION OF REMOTE CHEMICAL SYNAPSES
    CARNEVALE, NT
    JOHNSTON, D
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1982, 47 (04) : 606 - 621
  • [5] Comparative electrotonic analysis of three classes of rat hippocampal neurons
    Carnevale, NT
    Tsai, KY
    Claiborne, BJ
    Brown, TH
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1997, 78 (02) : 703 - 720
  • [6] CLAIBORNE BJ, 1992, METHODS NEUROSCIENCE, V10, P315
  • [7] Colbert CM, 1996, J NEUROSCI, V16, P6676
  • [8] Active dendrites reduce location-dependent variability of synaptic input trains
    Cook, EP
    Johnston, D
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1997, 78 (04) : 2116 - 2128
  • [9] Reliability and state dependence of pyramidal cell-interneuron synapses in the hippocampus:: an ensemble approach in the behaving rat
    Csicsvari, J
    Hirase, H
    Czurko, A
    Buzsáki, G
    [J]. NEURON, 1998, 21 (01) : 179 - 189
  • [10] Amplification of EPSPs by low Ni2+- and amiloride-sensitive Ca2+ channels in apical dendrites of rat CA1 pyramidal neurons
    Gillessen, T
    Alzheimer, C
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1997, 77 (03) : 1639 - 1643