Comparison of Two Nucleic Acid Amplification Assays, the Xpert MTB/RIF Assay and the Amplified Mycobacterium Tuberculosis Direct Assay, for Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens

被引：49

作者：

Teo, Jeanette ^{[1
]}

Jureen, Roland ^{[1
]}

Chiang, Donald ^{[1
]}

Chan, Douglas ^{[1
]}

Lin, Raymond ^{[1
]}

机构：

[1] Natl Univ Singapore Hosp, Dept Lab Med, Microbiol Unit, Singapore 119074, Singapore

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 10期

关键词：

RIFAMPIN RESISTANCE;

D O I：

10.1128/JCM.00211-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We compared the performance of the Xpert MTB/RIF assay, a new real-time tuberculosis (TB) PCR test, with that of the Amplified Mycobacterium Tuberculosis Direct (MTD) assay using 162 respiratory and nonrespiratory specimens. Based on culture as the gold standard, the overall sensitivity and specificity for all sample types for the Xpert MTB/RIF assay were 90.9 and 89%, respectively, while for the MTD assay, the overall sensitivity and specificity were 97.3 and 87.1%, respectively. A higher proportion of total equivocal results were obtained for the MTD assay, at 10.5% (17/162), while the Xpert MTB/RIF assay generated 5.5% (9/162) of invalid reads.

引用

页码：3659 / 3662

页数：4

共 9 条

[1]

[Anonymous], 2010 2011 TUB GLOB F

[2] Rapid Molecular Detection of Tuberculosis and Rifampin Resistance [J].

Boehme, Catharina C. ;

Nabeta, Pamela ;

Hillemann, Doris ;

Nicol, Mark P. ;

Shenai, Shubhada ;

Krapp, Fiorella ;

Allen, Jenny ;

Tahirli, Rasim ;

Blakemore, Robert ;

Rustomjee, Roxana ;

Milovic, Ana ;

Jones, Martin ;

O'Brien, Sean M. ;

Persing, David H. ;

Ruesch-Gerdes, Sabine ;

Gotuzzo, Eduardo ;

Rodrigues, Camilla ;

Alland, David ;

Perkins, Mark D. .

NEW ENGLAND JOURNAL OF MEDICINE, 2010, 363 (11) :1005-1015

[3]

Coll P, 2003, INT J TUBERC LUNG D, V7, P886

[4] Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand, Near-Patient Technology [J].

Helb, Danica ;

Jones, Martin ;

Story, Elizabeth ;

Boehme, Catharina ;

Wallace, Ellen ;

Ho, Ken ;

Kop, JoAnn ;

Owens, Michelle R. ;

Rodgers, Richard ;

Banada, Padmapriya ;

Safi, Hassan ;

Blakemore, Robert ;

Lan, N. T. Ngoc ;

Jones-Lopez, Edward C. ;

Levi, Michael ;

Burday, Michele ;

Ayakaka, Irene ;

Mugerwa, Roy D. ;

McMillan, Bill ;

Winn-Deen, Emily ;

Christel, Lee ;

Dailey, Peter ;

Perkins, Mark D. ;

Persing, David H. ;

Alland, David .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :229-237

[5]

METCHOCK B, 2007, MANUAL CLIN MICROBIO

[6] Rapid Detection of Mycobacterium tuberculosis Complex and Rifampin Resistance in Smear-Negative Clinical Samples by Use of an Integrated Real-Time PCR Method [J].

Moure, Raquel ;

Munoz, Laura ;

Torres, Miriam ;

Santin, Miguel ;

Martin, Rogelio ;

Alcaide, Fernando .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (03) :1137-1139

[7] Newer diagnostics for tuberculosis and multi-drug resistant tuberculosis [J].

Palomino, Juan Carlos .

CURRENT OPINION IN PULMONARY MEDICINE, 2006, 12 (03) :172-178

[8] Facing the crisis: Improving the diagnosis of tuberculosis in the HIV era [J].

Perkins, Mark D. ;

Cunningham, Jane .

JOURNAL OF INFECTIOUS DISEASES, 2007, 196 :S15-S27

[9] DETECTION OF RIFAMPICIN-RESISTANCE MUTATIONS IN MYCOBACTERIUM-TUBERCULOSIS [J].

TELENTI, A ;

IMBODEN, P ;

MARCHESI, F ;

LOWRIE, D ;

COLE, S ;

COLSTON, MJ ;

MATTER, L ;

SCHOPFER, K ;

BODMER, T .

LANCET, 1993, 341 (8846) :647-650

← 1 →