Cloning and sequence analysis of banana streak virus DNA

被引:114
作者
Harper, G [1 ]
Hull, R [1 ]
机构
[1] John Innes Ctr, Dept Virus Res, Norwich NR4 7UH, Norfolk, England
关键词
banana streak badnavirus; virus sequence; virus diagnosis; badnavirus phylogeny;
D O I
10.1023/A:1008021921849
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Banana streak virus (BSV), a member of the Badnavirus group of plant viruses, causes severe problems in banana cultivation, reducing fruit yield and restricting plant breeding and the movement of germplasm. Current detection methods are relatively insensitive. In order to develop a PCR-based diagnostic method that is both reliable and sensitive, the genome of a Nigerian isolate of BSV has been sequenced and shown to comprise 7389 bp and to be organized in a manner characteristic of badnaviruses. Comparison of this sequence with those of other badnaviruses showed that BSV is a distinct virus. PCR with primers based on sequence data indicated that BSV sequences are present in the banana genome.
引用
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页码:271 / 278
页数:8
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