Tetraethylammonium ions block the nicotinic cholinergic receptors of cochlear outer hair cells

Cochlear outer hair cells (OHCs) express nicotinic acetylcholine receptors (nAChRs) whose calcium permeability allow the activation of co-localized Ca2+-sensitive K+ channels (SK-type). The large organic cation tetraethylammonium (TEA) is known to block at millimolar concentration voltage-gated and Ca2+-activated K+ currents in OHCs. In the present study, we show that extracellular TEA blocked much more efficiently, at micromolar concentrations, ACh-evoked K+ currents in isolated guinea pig OHCs. The dose-inhibition curve indicated an IC50 of 60 muM, a value two orders of magnitude lower than the one reported on SK or BK channels. The site of the blocking action was on the extracellular side of the plasma membrane since 10 mM intracellular TEA did not prevent or change the characteristics of the ACh-evoked K+ current. The block of this K+ current in OHCs was mainly explained by a direct action of TEA at the nAChRs. Indeed, we demonstrated that extracellular TEA inhibited directly the ionotropic cation current flowing through the nAChRs (IC50 =30 muM). This study demonstrated for the first time that extracellular TEA is an effective blocker of the OHCs' nAChRs. (C) 2001 Elsevier Science BM All rights reserved.