Alternative RNA splicing of the NMDA receptor NR1 mRNA in the neurons of the teleost electrosensory system

被引:29
作者
Bottai, D
Maler, L
Dunn, RJ
机构
[1] Montreal Gen Hosp, Res Inst, Neurosci Res Ctr, Montreal, PQ H3G 1A4, Canada
[2] Univ Ottawa, Dept Anat & Neurobiol, Ottawa, ON K1H 8M5, Canada
[3] McGill Univ, Dept Biol, Montreal, PQ H3G 1A4, Canada
[4] McGill Univ, Dept Neurol, Montreal, PQ H3G 1A4, Canada
关键词
NMDA receptor; electrosensory system; NR1; subunit; RNA splicing; evolution; fish neurons;
D O I
10.1523/JNEUROSCI.18-14-05191.1998
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The sequence for cDNA encoding the NMDA receptor subunit 1 (aptNR1) of the weakly electric fish Apteronotus leptorhynchus has been determined. The deduced amino acid sequence is similar to 88% identical to other vertebrate NR1 proteins, with sequence homology extending to the alternatively spliced cassettes N1 and C1. The fish and mammalian N1 and C1 splice cassettes are identical at 20 of 21 and 30 of 37 amino acid positions, respectively. We did not detect a C2 splice cassette in aptNR1 mRNA, but we did find two novel C-terminal alternative splice cassettes labeled C1' and C1". The relative levels of NR1 transcripts containing the N1 and C1 splice cassettes were determined by using RNase protection and in situ hybridization analysis. N1-containing mRNAs are more abundant in cauda[ brain regions, similar to the pat terns reported for mammalian brain. In contrast, the relative levels of transcripts containing the C1 splice cassette are much lower in fish than in mammals, averaging only 9% for the whole brain. The levels of C1 splicing increased in more rostra[ brain regions. In situ hybridizations with N1- and C1-specific probes demonstrated that N1 cassette splicing occurs in most neurons but that C1 splicing is heterogeneous and is restricted to a subset of neuronal types in the electrosensory system.
引用
收藏
页码:5191 / 5202
页数:12
相关论文
共 75 条
[1]   COMBINATORIAL RNA SPLICING ALTERS THE SURFACE-CHARGE ON THE NMDA RECEPTOR [J].
ANANTHARAM, V ;
PANCHAL, RG ;
WILSON, A ;
KOLCHINE, VV ;
TREISTMAN, SN ;
BAYLEY, H .
FEBS LETTERS, 1992, 305 (01) :27-30
[2]   THE ROLE OF AMINO-ACID NEUROTRANSMITTERS IN THE DESCENDING CONTROL OF ELECTRORECEPTION [J].
BASTIAN, J .
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 1993, 172 (04) :409-423
[3]  
BASTIAN J, 1995, J COMP PHYSIOL A, V176, P63
[4]   Plasticity in an electrosensory system .1. General features of a dynamic sensory filter [J].
Bastian, J .
JOURNAL OF NEUROPHYSIOLOGY, 1996, 76 (04) :2483-2496
[5]   Plasticity in an electrosensory system .2. Postsynaptic events associated with a dynamic sensory filter [J].
Bastian, J .
JOURNAL OF NEUROPHYSIOLOGY, 1996, 76 (04) :2497-2507
[6]   Excitatory amino acid receptors at a feedback pathway in the electrosensory system: Implications for the searchlight hypothesis [J].
Berman, NJ ;
Plant, J ;
Turner, RW ;
Maler, L .
JOURNAL OF NEUROPHYSIOLOGY, 1997, 78 (04) :1869-1881
[7]  
Bottai D, 1997, J COMP NEUROL, V389, P65, DOI 10.1002/(SICI)1096-9861(19971208)389:1<65::AID-CNE5>3.0.CO
[8]  
2-C
[9]  
BULLER AL, 1994, J NEUROSCI, V14, P5471
[10]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2