Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar Candida screening and susceptibility testing of isolates

Candida dubliniensis has been associated with oropharyngeal candidiasis in patients infected with human immunodeficiency virus (HIV), C, dubliniensis isolates may have: been improperly characterized as atypical Candida albicans due to the phenotypic similarity between the two species. Prospective screening of oral rinses from 63 HIV-infected patients detected atypical dark green isolates on CHROMagar Candida compared to typical C, albicans isolates, which are light green, Forty-eight atypical isolates and three control strains were characterized by germ tube formation, differential growth at 37, 42, and 45 degrees C, identification by API 20C, fluorescence, chlamydoconidium production, and fingerprinting by Ca3 probe DNA hybridization patterns. All isolates were germ tube positive, Very poor or no growth occurred at 42 degrees C with 22 of 51 isolates. All 22 poorly growing isolates at 42 degrees C and one isolate with growth at 42 degrees C showed weak hybridization of the Ca3 probe with genomic DNA, consistent with C. dubliniensis identification. No C. dubliniensis isolate but only 18 of 28C, albicans isolates grew at 45 degrees C, Other phenotypic or morphologic tests were less reliable in differentiating C, dubliniensis from C, albicans, Antifungal susceptibility testing showed fluconazole MICs ranging from less than or equal to 0.125 to 64 mu g/ml. Two isolates were resistant to fluconazale (MIC, 64 mu g/ml) and one strain was dose dependent susceptible (MIC, 16 mu g/ml). MICs of other azoles, including voriconazole, itraconazole, and SCH 56592, for these isolates were lower. C, dubliniensis was identified in 11 of 63 (17%) serially evaluated patients, Variability in phenotypic characteristics dictates the use of molecular and biochemical techniques to identify C, dubliniensis, This study identifies C, dubliniensis in HIV-infected patients from San Antonio, Tex,, and shows that C, dubliniensis is frequently detected in those patients by using a primary CHROMagar screen.