Haploid plantlet screening in the development of blackleg resistant DH lines of Brassica napus

被引：5

作者：

Bansal, VK ^{[1
]}

Thiagarajah, MR ^{[1
]}

Stringam, GR ^{[1
]}

Hardin, RT ^{[1
]}

机构：

[1] Univ Alberta, Dept Agr Food & Nutrit Sci, Fac Agr Forestry & Home Econ, Agr Forestry Ctr 410, Edmonton, AB T6G 2P5, Canada

来源：

PLANT BREEDING | 1998年 / 117卷 / 02期

关键词：

Brassica napus; Leptosphaeria maculans; blackleg; breeding; doubled haploid lines; haploid plantlet screening; resistance;

D O I：

10.1111/j.1439-0523.1998.tb01460.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 [作物学];

摘要：

This study was conducted to expedite disease-resistance breeding in canola by screening haploid plants against blackleg disease. Microspore-derived haploid plantlets from 14 unrelated crosses were inoculated with Leptosphaeria maculans pycnidiospores and were rated as resistant, intermediate or susceptible. Blackleg-inoculated and control haploid plants were then colchicine-treated to produce doubled haploid (DH) lines. The DH lines thus produced were again screened against blackleg using the cotyledon bioassay. In general, the proportion of the resistant DH lines derived from selected resistant haploid plants was much higher than the proportion of resistant DH lines from control haploid populations. There was no detrimental effect on the survival of haploid plants after the combined treatment of blackleg inoculation and colchicine. The importance of the micro-environment on the final outcome of plant-pathogen interaction in case of non-obligate pathogens is also discussed.

引用

页码：103 / 106

页数：4

共 17 条

[1]

Agrios, 1988, PLANT PATHOL, P40, DOI [10.1016/b978-0-12-044563-9.50006-2, DOI 10.1016/B978-0-12-044563-9.50006-2]

[2]

A COMPARISON OF GREENHOUSE AND FIELD SCREENING METHODS FOR BLACKLEG RESISTANCE IN DOUBLED HAPLOID LINES OF BRASSICA-NAPUS [J].