High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

被引：8

作者：

Krishnamoorthy, Ganeshram ^{[1
]}

Beusink, J. Bianca ^{[1
]}

Schasfoort, Richard B. M. ^{[1
]}

机构：

[1] Univ Twente, MESA Inst Nanotechnol, BIOS Lab on a Chip Grp, NL-7500 AE Enschede, Netherlands

来源：

ANALYTICAL METHODS | 2010年 / 2卷 / 08期

关键词：

OPTICAL BIOSENSOR; BINDING-KINETICS; DNA;

D O I：

10.1039/c0ay00112k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper we show a high-throughput method to screen the kinetics and affinity constants of many biomolecular interactions simultaneously. During the preparation of the sensor chip, ligands were serially diluted and spotted in a 6 x 4 microarray on the sensor surface. A multi-analyte sample was injected and the real-time surface plasmon resonance (SPR) responses of all the 24 microarray spots were obtained using a commercial SPR imaging instrument. The multi responses of the association and dissociation processes obtained from a single analyte injection are sufficient to calculate the rates and affinity constants of the interactions between three interactant antigen-antibody pairs using a simple monophasic kinetic model. The method drastically reduces the measurement time and cost in the benefit of increased throughput.

引用

页码：1020 / 1025

页数：6

共 25 条

[1] One hundred spots parallel monitoring of DNA interactions by SPR imaging of polymer-functionalized surfaces applied to the detection of cystic fibrosis mutations [J].