Allatostatin gene expression in brain and midgut, and activity of synthetic allatostatins on feeding-related processes in the cockroach Blattella germanica

被引：52

作者：

Aguilar, R ^{[1
]}

Maestro, JL ^{[1
]}

Vilaplana, L ^{[1
]}

Pascual, N ^{[1
]}

Piulachs, MD ^{[1
]}

Bellés, X ^{[1
]}

机构：

[1] CSIC, Inst Biol Mol Barcelona, Dept Physiol & Mol Biodivers, ES-08034 Barcelona, Spain

来源：

REGULATORY PEPTIDES | 2003年 / 115卷 / 03期

关键词：

allatostatin; Blattella germanica; German cockroach; gene expression; juvenile hormone; food intake control;

D O I：

10.1016/S0167-0115(03)00165-4

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Allatostatins of the YXFGLamide group were discovered in cockroaches through their capacity to inhibit juvenile hormone biosynthesis. Here, we assess the occurrence of preproallatostatin (preproAST) mRNA in the brain and midgut of adult females of the cockroach Blattella germanica, and estimate brain and midgut preproAST mRNA levels during the first reproductive cycle. Reverse transcription polymerase chain reaction (RT-PCR) shows that brain preproAST mRNA levels increase slightly during the gonadotrophic cycle, and remain high during ootheca transport. In the midgut, preproAST mRNA levels decline around the middle of the gonadotrophic cycle. The pattern of allatostatin expression in gut tissues suggests that these peptides play roles related to feeding and nutrition. Our results have shown that synthetic allatostatins inhibit hindgut motility and activate midgut alpha-amylase secretion. In addition, injected allatostatins inhibit food consumption, which might be connected to the above activities. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：171 / 177

页数：7

共 38 条

[1] INVITRO BIOSYNTHESIS OF JH-III BY THE CORPORA ALLATA OF ADULT FEMALES OF BLATTELLA-GERMANICA (L) [J].

BELLES, X ;

CASAS, J ;

MESSEGUER, A ;

PIULACHS, MD .

INSECT BIOCHEMISTRY, 1987, 17 (07) :1007-1010

[2] The molecular evolution of the allatostatin precursor in cockroaches [J].

Bellés, X ;

Graham, LA ;

Bendena, WG ;

Ding, Q ;

Edwards, JP ;

Weaver, RJ ;

Tobe, SS .

PEPTIDES, 1999, 20 (01) :11-22

[3] ALLATOSTATIC NEUROPEPTIDES FROM THE COCKROACH BLATTELLA-GERMANICA (L) (DICTYOPTERA, BLATTELLIDAE) - IDENTIFICATION, IMMUNOLOCALIZATION AND ACTIVITY [J].

BELLES, X ;

MAESTRO, JL ;

PIULACHS, MD ;

JOHNSEN, AH ;

DUVE, H ;

THORPE, A .

REGULATORY PEPTIDES, 1994, 53 (03) :237-247

[4] Allatostatins: Diversity in structure and function of an insect neuropeptide family [J].

Bendena, WG ;

Garside, CS ;

Yu, CG ;

Tobe, SS .

NEUROPEPTIDES IN DEVELOPMENT AND AGING, 1997, 814 :53-66

[5] Partial characterization of a putative allatostatin receptor in the midgut of the cockroach Diploptera punctata [J].

Bowser, PRF ;

Tobe, SS .

GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2000, 119 (01) :1-10

[6] Characterisation of the helicostatin peptide precursor gene from Helicoverpa armigera (Lepidoptera: Noctuidae) [J].

Davey, M ;

Duve, H ;

Thorpe, A ;

East, P .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 29 (12) :1119-1127

[7] Comparison of the allatostatin neuropeptide precursors in the distantly related cockroaches Periplaneta americana and Diploptera punctata [J].

Ding, Q ;

Donly, BC ;

Tobe, SS ;

Bendena, WG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 234 (03) :737-746

[8] Structure, distribution, and biological activity of novel members of the allatostatin family in the crayfish Orconectes limosus [J].

Dircksen, H ;

Skiebe, P ;

Abel, B ;

Agricola, H ;

Buchner, K ;

Muren, JE ;

Nässel, DR .

PEPTIDES, 1999, 20 (06) :695-712

[9] MOLECULAR-CLONING OF THE GENE FOR THE ALLATOSTATIN FAMILY OF NEUROPEPTIDES FROM THE COCKROACH DIPLOPTERA-PUNCTATA [J].

DONLY, BC ;

DING, Q ;

TOBE, SS ;

BENDENA, WG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (19) :8807-8811

[10] Identification of the dipteran Leu-callatostatin peptide family: The pattern of precursor processing revealed by isolation studies in Calliphora vomitoria [J].

Duve, H ;

Johnsen, AH ;

Maestro, JL ;

Scott, AG ;

East, PD ;

Thorpe, A .

REGULATORY PEPTIDES, 1996, 67 (01) :11-19

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