Obesity-related differential gene expression in the visceral adipose tissue

被引:56
作者
Baranova, A
Collantes, R
Gowder, SJ
Elariny, H
Schlauch, K
Younoszai, A
King, S
Randhawa, M
Pusulury, S
Alsheddi, T
Ong, JP
Martin, LM
Chandhoke, V
Younossi, ZM
机构
[1] Inova Fairfax Hosp, Ctr Liver Dis, Falls Church, VA 22042 USA
[2] George Mason Univ, Ctr Study Genom Liver Dis, Mol & Microbiol Dept, Fairfax, VA 22030 USA
关键词
morbid obesity; adipose tissue; microarrays; gene expression;
D O I
10.1381/0960892054222876
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: This study investigates the expression patterns in human adipose tissue, and identifies genes that may be involved in the abnormal energy homeostasis. Methods: Subjects were prospectively recruited from morbidly obese patients undergoing bariatric surgery and from non-obese organ donors. Extensive clinical data and visceral fat specimens were obtained from each subject at the time of surgery. A group of 50 obese patients and 9 non-obese controls were selected for further study. Two custom two-color cDNA microarrays were produced with 40,173 human individual cDNA clones. Microarray experiments were performed for each sample, and a selected group of gene expression values were confirmed with real-time RT-PCR. Results: A comparison of gene expression profiles from obese and non-obese patients identified 1,208 genes with statistically significant differential expression between the 2 groups. Most prominent among these genes are multiple glycolysis enzyme encoding genes; others are involved in oxysterol biosynthesis and signaling, or are ATP-binding transporters and solute carriers. Conclusion: Differential gene expression in the adipose tissue of morbidly obese patients includes genes related to lipid and glucose metabolism, membrane transport, and genes promoting the cell cycle. These findings are a first step toward clarifying the molecular pathogenesis of obesity and identifying potential targets for therapeutic intervention.
引用
收藏
页码:758 / 765
页数:8
相关论文
共 19 条
[1]   Distinct organization of the candidate tumor suppressor gene RFP2 in human and mouse:: multiple mRNA isoforms in both species- and human-specific antisense transcript RFP2OS [J].
Baranova, A ;
Hammarsund, M ;
Ivanova, DV ;
Skoblov, M ;
Sangfelt, O ;
Corcoran, M ;
Borodina, T ;
Makeeva, N ;
Pestova, A ;
Tyazhelova, T ;
Nazarenko, S ;
Gorreta, F ;
Alsheddi, T ;
Schlauch, K ;
Nikitin, E ;
Kapanadze, B ;
Shagin, D ;
Poltaraus, A ;
Vorobiev, AI ;
Zabarovsky, E ;
Lukianov, S ;
Chandhoke, V ;
Ibbotson, R ;
Oscier, D ;
Einhorn, S ;
Grander, D ;
Yankovsky, N .
GENE, 2003, 321 :103-112
[2]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[3]   Cluster analysis and display of genome-wide expression patterns [J].
Eisen, MB ;
Spellman, PT ;
Brown, PO ;
Botstein, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14863-14868
[4]   Numerical methods for handling uncertainty in microarray data: An example analyzing perturbed mitochondrial function in yeast [J].
Epstein, CB ;
Hale, W ;
Butow, RA .
METHODS IN CELL BIOLOGY, VOL 65, 2001, 65 :439-452
[5]  
Forest C, 2003, BIOCHEM SOC T, V31, P1125
[6]   Molecular characterization of a local sulfonylurea system in human adipose tissue [J].
Gabrielsson, BG ;
Karlsson, AC ;
Lönn, M ;
Olofsson, LE ;
Johansson, JM ;
Torgerson, JS ;
Sjöström, L ;
Carlsson, B ;
Edén, S ;
Carlsson, LMS .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2004, 258 (1-2) :65-71
[7]  
GOLAY A, 1994, DIABETES METAB, V20, P3
[8]   Gene expression profile of omental adipose tissue in human obesity [J].
Gómez-Ambrosi, J ;
Catalán, V ;
Diez-Caballero, A ;
Martínez-Cruz, LA ;
Gil, MJ ;
García-Foncillas, J ;
Cienfuegoas, JA ;
Salvador, J ;
Mato, JM ;
Fruhbeck, G .
FASEB JOURNAL, 2004, 18 (01) :215-+
[9]   25-hydroxycholesterol acts in the Golgi compartment to induce degradation of tyrosinase [J].
Hall, AM ;
Krishnamoorthy, L ;
Orlow, SJ .
PIGMENT CELL RESEARCH, 2004, 17 (04) :396-406
[10]  
Jong MC, 2001, J LIPID RES, V42, P1578