Molecular and functional characterization of chicken IL-15

被引:34
作者
Choi, KD
Lillehoj, HS
Song, KD
Han, JY
机构
[1] USDA, Immunol Dis Resistance Lab, Inst Livestock & Poultry Sci, Beltsville, MD 20705 USA
[2] Seoul Natl Univ, Coll Agr & Life Sci, Dept Anim Sci & Technol, Suwon 441744, South Korea
关键词
interleukin-15; gamma delta TCR T cells; cytokine; T cell growth factor; chicken cytokine;
D O I
10.1016/S0145-305X(98)00046-9
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A cDNA encoding chicken interleukin-15 was cloned from a CD4(+) T cell hybridoma expression library by screening with a rabbit antibody against a protein fraction of conditioned medium containing T cell growth promoting activity. The chicken IL-15 cDNA contains an open reading frame of 143 amino acids with a single potential N-linked glycosylation site. The predicted m.w. of the encoded protein (16 kDa) matched the size of an immunoreactive band on Western blots of E. coli expressing the recombinant IL-15. Amino acid and nucleotide sequence analyses of chicken IL-15 revealed 31% and 46% identity with bovine IL-15 respectively and lesser homologies to other mammalian IL-15s. Chicken IL-15 contained all 4 highly conserved cysteine residues present in mammalian IL-15 sequences. RT-PCR demonstrated that the chicken IL-15 gene is expressed in many tissues including spleen, intestine, and muscle and in established macrophage. T lymphoma and fibroblast cell lines. Activation of spleen cells with Con A enhanced the expression of IL-15 gene transcripts in a time-dependent manner. CHO-KI cells transfected with the chicken IL-15 cDNA secreted a biologically active protein supporting the growth of Con A activated spleen lymphocytes. Continuous culture of spleen Con A lymphoblasts with chicken IL-15 over two months resulted in an enriched T lymphocyte population expressing the gamma delta TCR, CD8 alpha, and CD3 cell surface antigens. Published by Elsevier Science Ltd.
引用
收藏
页码:165 / 177
页数:13
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