Isolation and characterisation of Shiga toxin-producing Escherichia coli O157:H7 from calves in Argentina

被引:13
作者
Chinen, I
Otero, JL
Miliwebsky, ES
Roldán, ML
Baschkier, A
Chillemi, GM
Nóboli, C
Frizzo, L
Rivas, M
机构
[1] Univ Nacl Litoral, Dept Salud Publ Vet, Fac Cs Vet, RA-2805 Esperanza, Argentina
[2] ANLIS Dr Carlos G Malbran, Serv Fisiopatogenia, Inst Nacl Enfermedades Infecciosas, Buenos Aires, DF, Argentina
关键词
cattle; clonal relatedness; haemolytic uraemic syndrome; STEC O157 : H7; virulence factors;
D O I
10.1016/S0034-5288(02)00192-3
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Four hundred and twenty-two calves were examined for intestinal carriage of Shiga toxin-producing Escherichia coli O157:H7 using conventional plating. Two (0.5%) E coli O157 were recovered. They were compared with 96 Argentine strains of different origin by pulsed-field gel electrophoresis, phage typing and PCR-RFLP of stx2 genes. One strain isolated from a calf, was closely related with 18 strains of clinical origin. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:283 / 286
页数:4
相关论文
共 20 条
[1]   Emerging foodborne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world [J].
Armstrong, GL ;
Hollingsworth, J ;
Morris, JG .
EPIDEMIOLOGIC REVIEWS, 1996, 18 (01) :29-51
[2]  
Balows A., 1991, MANUAL CLIN MICROBIO, P360
[3]   Prevalence and characteristics of Escherichia coli serotype O157:H7 and other verotoxin-producing E-coli in healthy cattle [J].
Blanco, M ;
Blanco, JE ;
Blanco, J ;
Gonzalez, EA ;
Mora, A ;
Prado, C ;
Fernandez, L ;
Rio, M ;
Ramos, J ;
Alonso, MP .
EPIDEMIOLOGY AND INFECTION, 1996, 117 (02) :251-257
[4]   Isolation and characterization of Escherichia coli O157:H7 from retail meats in Argentina [J].
Chinen, I ;
Tanaro, JD ;
Miliwebsky, E ;
Lound, LH ;
Chillemi, G ;
Ledri, S ;
Baschkier, A ;
Scarpin, M ;
Manfredi, E ;
Rivas, M .
JOURNAL OF FOOD PROTECTION, 2001, 64 (09) :1346-1351
[5]  
*COM NEFR, 1995, ARCH ARG PEDIAT, V93, P409
[6]   THE EPIDEMIOLOGY OF INFECTIONS CAUSED BY ESCHERICHIA-COLI O157-H7, OTHER ENTEROHEMORRHAGIC ESCHERICHIA-COLI, AND THE ASSOCIATED HEMOLYTIC UREMIC SYNDROME [J].
GRIFFIN, PM ;
TAUXE, RV .
EPIDEMIOLOGIC REVIEWS, 1991, 13 :60-98
[7]   Isolation and characterization of verocytotoxin-producing Escherichia coli O157 strains from Dutch cattle and sheep [J].
Heuvelink, AE ;
van den Biggelaar, FLAM ;
de Boer, E ;
Herbes, RG ;
Melchers, WJG ;
Huis In 'T Veld, JHJ ;
Monnens, LAH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) :878-882
[8]   SENSITIVE METHOD FOR DETECTING LOW NUMBERS OF VEROTOXIN-PRODUCING ESCHERICHIA-COLI IN MIXED CULTURES BY USE OF COLONY SWEEPS AND POLYMYXIN EXTRACTION OF VEROTOXIN [J].
KARMALI, MA ;
PETRIC, M ;
LIM, C ;
CHEUNG, R ;
ARBUS, GS .
JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (04) :614-619
[9]   Molecular epidemiology of Escherichia coli O157:H7 by pulsed-field gel electrophoresis and comparison with that by bacteriophage typing [J].
Krause, U ;
ThomsonCarter, FM ;
Pennington, TH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (04) :959-961
[10]  
McDonough PL, 2000, J CLIN MICROBIOL, V38, P318