Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva

被引：199

作者：

Ogawa, Yuko ^{[2
]}

Miura, Yuri ^{[3
]}

Harazono, Akira ^{[4
]}

Kanai-Azuma, Masami ^{[5
]}

Akimoto, Yoshihiro ^{[5
]}

Kawakami, Hayato ^{[5
]}

Yamaguchi, Teruhide ^{[4
]}

Toda, Tosifusa ^{[3
]}

Endo, Tamao ^{[3
]}

Tsubuki, Masayoshi ^{[6
]}

Yanoshita, Ryohei ^{[1
]}

机构：

[1] Hoshi Univ, Dept Biol, Shinagawa Ku, Tokyo 1428591, Japan

[2] Teikyo Heisei Univ, Fac Pharmaceut Sci, Chiba 2900193, Japan

[3] Tokyo Metropolitan Inst Gerontol, Res Team Mech Aging, Itabashi Ku, Tokyo 1730015, Japan

[4] Natl Inst Hlth Sci, Div Biol Chem & Biol, Setagaya Ku, Tokyo 1588591, Japan

[5] Kyorin Univ, Sch Med, Dept Anat, Tokyo 1818611, Japan

[6] Hoshi Univ, Inst Med Chem, Tokyo 1428591, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2011年 / 34卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

human whole saliva; exosome; proteome; dipeptidyl peptidase IV; DIPEPTIDYL-PEPTIDASE-IV; CELL-DERIVED EXOSOMES; SJOGRENS-SYNDROME; DENDRITIC CELLS; VESICLES; PROTEIN; EXPRESSION; GLANDS; LYMPHOCYTES; IDENTIFICATION;

D O I：

10.1248/bpb.34.13

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Saliva contains a large number of proteins that participate in the protection of oral tissue. Exosomes are small vesicles (30-100 urn in diameter) with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We have recently demonstrated that exosomes are present in human whole saliva. In this study, we found that whole saliva contained at least two types of exosomes (exosome I and exosome II) that are different in size and protein composition. Proteomic analysis revealed that both types of exosomes contained Alix, Tsg101 and Hsp70, all exosomal markers, immunoglobulin A and polymeric immunoglobulin receptor, whereas they had different protein compositions. Most of dipeptidyl peptidase IV known as CD26 in whole saliva, was present on the exosome II and metabolically active in cleaving chemokines (CXCL11 and CXCL12). Human whole saliva exosomes might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.

引用

页码：13 / 23

页数：11

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