Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA

被引：340

作者：

Szwagierczak, Aleksandra ^{[1
]}

Bultmann, Sebastian ^{[1
]}

Schmidt, Christine S. ^{[1
]}

Spada, Fabio ^{[1
]}

Leonhardt, Heinrich ^{[1
]}

机构：

[1] Univ Munich, Ctr Integrated Prot Sci Munich, Dept Biol, D-82152 Planegg Martinsried, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2010年 / 38卷 / 19期

关键词：

ACUTE MYELOID-LEUKEMIA; DEOXYRIBONUCLEIC ACID; MLL; T(10/11)(Q22; Q23); 5-METHYLCYTOSINE; METHYLATION; ENZYMES; PROTEIN; NEURONS; DNMT3A;

D O I：

10.1093/nar/gkq684

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.

引用

页数：5

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